var bibbase_data = {"data":"<img src=\"//bibbase.org/img/ajax-loader.gif\" id=\"spinner\"\n  style=\"display: none;\" alt=\"Loading..\" />\n\n<div id=\"bibbase\">\n\n  <script type=\"text/javascript\">\n    var bibbase = {\n      params: {\"bib\":\"https://api.zotero.org/users/3476755/collections/IHG8YRKP/items?key=VpWpxx7vAcI1RFC3pAUNOY4B&format=bibtex&limit=100\",\"jsonp\":\"1\",\"authorFirst\":\"1\",\"hidemenu\":\"true\",\"commas\":\"true\",\"sort\":\"author_short\",\"groupby\":\"\",\"filter\":\"authors:(Inaba|Shiba|稲葉|柴)\",\"host\":\"bibbase.org\"},\n      data: [{\"bibtype\":\"article\",\"type\":\"article\",\"title\":\"A new species of Acoela possessing a middorsal appendage with a possible sensory function\",\"volume\":\"39\",\"issn\":\"0289-0003\",\"url\":\"https://bioone.org/journals/zoological-science/volume-39/issue-1/zs210058/A-New-Species-of-Acoela-Possessing-a-Middorsal-Appendage-with/10.2108/zs210058.full\",\"doi\":\"10.2108/zs210058\",\"number\":\"1\",\"urldate\":\"2022-01-25\",\"journal\":\"Zoological Science\",\"author\":[{\"propositions\":[],\"lastnames\":[\"Asai\"],\"firstnames\":[\"Masashi\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Miyazawa\"],\"firstnames\":[\"Hideyuki\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Yanase\"],\"firstnames\":[\"Ryuji\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Inaba\"],\"firstnames\":[\"Kazuo\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Nakano\"],\"firstnames\":[\"Hiroaki\"],\"suffixes\":[]}],\"month\":\"January\",\"year\":\"2022\",\"pages\":\"147–156\",\"bibtex\":\"@article{asai_new_2022,\\n\\ttitle = {A new species of {Acoela} possessing a middorsal appendage with a possible sensory function},\\n\\tvolume = {39},\\n\\tissn = {0289-0003},\\n\\turl = {https://bioone.org/journals/zoological-science/volume-39/issue-1/zs210058/A-New-Species-of-Acoela-Possessing-a-Middorsal-Appendage-with/10.2108/zs210058.full},\\n\\tdoi = {10.2108/zs210058},\\n\\tnumber = {1},\\n\\turldate = {2022-01-25},\\n\\tjournal = {Zoological Science},\\n\\tauthor = {Asai, Masashi and Miyazawa, Hideyuki and Yanase, Ryuji and Inaba, Kazuo and Nakano, Hiroaki},\\n\\tmonth = jan,\\n\\tyear = {2022},\\n\\tpages = {147--156},\\n}\\n\\n\",\"author_short\":[\"Asai, M.\",\"Miyazawa, H.\",\"Yanase, R.\",\"Inaba, K.\",\"Nakano, H.\"],\"key\":\"asai_new_2022\",\"id\":\"asai_new_2022\",\"bibbaseid\":\"asai-miyazawa-yanase-inaba-nakano-anewspeciesofacoelapossessingamiddorsalappendagewithapossiblesensoryfunction-2022\",\"role\":\"author\",\"urls\":{\"Paper\":\"https://bioone.org/journals/zoological-science/volume-39/issue-1/zs210058/A-New-Species-of-Acoela-Possessing-a-Middorsal-Appendage-with/10.2108/zs210058.full\"},\"metadata\":{\"authorlinks\":{\"inaba, k\":\"https://www.shimoda.tsukuba.ac.jp/en/staff_inaba.html\"}},\"downloads\":36,\"html\":\"\"},{\"bibtype\":\"article\",\"type\":\"article\",\"title\":\"Mass spectrometry of short peptides reveals common features of metazoan peptidergic neurons\",\"volume\":\"6\",\"issn\":\"2397-334X\",\"url\":\"https://www.nature.com/articles/s41559-022-01835-7\",\"doi\":\"10.1038/s41559-022-01835-7\",\"abstract\":\"Abstract The evolutionary origins of neurons remain unknown. Although recent genome data of extant early-branching animals have shown that neural genes existed in the common ancestor of animals, the physiological and genetic properties of neurons in the early evolutionary phase are still unclear. Here, we performed a mass spectrometry-based comprehensive survey of short peptides from early-branching lineages Cnidaria, Porifera and Ctenophora. We identified a number of mature ctenophore neuropeptides that are expressed in neurons associated with sensory, muscular and digestive systems. The ctenophore peptides are stored in vesicles in cell bodies and neurites, suggesting volume transmission similar to that of cnidarian and bilaterian peptidergic systems. A comparison of genetic characteristics revealed that the peptide-expressing cells of Cnidaria and Ctenophora express the vast majority of genes that have pivotal roles in maturation, secretion and degradation of neuropeptides in Bilateria. Functional analysis of neuropeptides and prediction of receptors with machine learning demonstrated peptide regulation of a wide range of target effector cells, including cells of muscular systems. The striking parallels between the peptidergic neuronal properties of Cnidaria and Bilateria and those of Ctenophora, the most basal neuron-bearing animals, suggest a common evolutionary origin of metazoan peptidergic nervous systems.\",\"language\":\"en\",\"number\":\"10\",\"urldate\":\"2022-10-25\",\"journal\":\"Nature Ecology & Evolution\",\"author\":[{\"propositions\":[],\"lastnames\":[\"Hayakawa\"],\"firstnames\":[\"Eisuke\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Guzman\"],\"firstnames\":[\"Christine\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Horiguchi\"],\"firstnames\":[\"Osamu\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Kawano\"],\"firstnames\":[\"Chihiro\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Shiraishi\"],\"firstnames\":[\"Akira\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Mohri\"],\"firstnames\":[\"Kurato\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Lin\"],\"firstnames\":[\"Mei-Fang\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Nakamura\"],\"firstnames\":[\"Ryotaro\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Nakamura\"],\"firstnames\":[\"Ryo\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Kawai\"],\"firstnames\":[\"Erina\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Komoto\"],\"firstnames\":[\"Shinya\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Jokura\"],\"firstnames\":[\"Kei\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Shiba\"],\"firstnames\":[\"Kogiku\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Shigenobu\"],\"firstnames\":[\"Shuji\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Satake\"],\"firstnames\":[\"Honoo\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Inaba\"],\"firstnames\":[\"Kazuo\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Watanabe\"],\"firstnames\":[\"Hiroshi\"],\"suffixes\":[]}],\"month\":\"August\",\"year\":\"2022\",\"pages\":\"1438–1448\",\"bibtex\":\"@article{hayakawa_mass_2022,\\n\\ttitle = {Mass spectrometry of short peptides reveals common features of metazoan peptidergic neurons},\\n\\tvolume = {6},\\n\\tissn = {2397-334X},\\n\\turl = {https://www.nature.com/articles/s41559-022-01835-7},\\n\\tdoi = {10.1038/s41559-022-01835-7},\\n\\tabstract = {Abstract\\n            The evolutionary origins of neurons remain unknown. Although recent genome data of extant early-branching animals have shown that neural genes existed in the common ancestor of animals, the physiological and genetic properties of neurons in the early evolutionary phase are still unclear. Here, we performed a mass spectrometry-based comprehensive survey of short peptides from early-branching lineages Cnidaria, Porifera and Ctenophora. We identified a number of mature ctenophore neuropeptides that are expressed in neurons associated with sensory, muscular and digestive systems. The ctenophore peptides are stored in vesicles in cell bodies and neurites, suggesting volume transmission similar to that of cnidarian and bilaterian peptidergic systems. A comparison of genetic characteristics revealed that the peptide-expressing cells of Cnidaria and Ctenophora express the vast majority of genes that have pivotal roles in maturation, secretion and degradation of neuropeptides in Bilateria. Functional analysis of neuropeptides and prediction of receptors with machine learning demonstrated peptide regulation of a wide range of target effector cells, including cells of muscular systems. The striking parallels between the peptidergic neuronal properties of Cnidaria and Bilateria and those of Ctenophora, the most basal neuron-bearing animals, suggest a common evolutionary origin of metazoan peptidergic nervous systems.},\\n\\tlanguage = {en},\\n\\tnumber = {10},\\n\\turldate = {2022-10-25},\\n\\tjournal = {Nature Ecology \\\\& Evolution},\\n\\tauthor = {Hayakawa, Eisuke and Guzman, Christine and Horiguchi, Osamu and Kawano, Chihiro and Shiraishi, Akira and Mohri, Kurato and Lin, Mei-Fang and Nakamura, Ryotaro and Nakamura, Ryo and Kawai, Erina and Komoto, Shinya and Jokura, Kei and Shiba, Kogiku and Shigenobu, Shuji and Satake, Honoo and Inaba, Kazuo and Watanabe, Hiroshi},\\n\\tmonth = aug,\\n\\tyear = {2022},\\n\\tpages = {1438--1448},\\n}\\n\\n\",\"author_short\":[\"Hayakawa, E.\",\"Guzman, C.\",\"Horiguchi, O.\",\"Kawano, C.\",\"Shiraishi, A.\",\"Mohri, K.\",\"Lin, M.\",\"Nakamura, R.\",\"Nakamura, R.\",\"Kawai, E.\",\"Komoto, S.\",\"Jokura, K.\",\"Shiba, K.\",\"Shigenobu, S.\",\"Satake, H.\",\"Inaba, K.\",\"Watanabe, H.\"],\"key\":\"hayakawa_mass_2022\",\"id\":\"hayakawa_mass_2022\",\"bibbaseid\":\"hayakawa-guzman-horiguchi-kawano-shiraishi-mohri-lin-nakamura-etal-massspectrometryofshortpeptidesrevealscommonfeaturesofmetazoanpeptidergicneurons-2022\",\"role\":\"author\",\"urls\":{\"Paper\":\"https://www.nature.com/articles/s41559-022-01835-7\"},\"metadata\":{\"authorlinks\":{\"inaba, k\":\"https://www.shimoda.tsukuba.ac.jp/en/staff_inaba.html\"}},\"html\":\"\"},{\"bibtype\":\"article\",\"type\":\"article\",\"title\":\"Fertilization modes and the evolution of sperm characteristics in marine fishes: Paired comparisons of externally and internally fertilizing species\",\"volume\":\"12\",\"issn\":\"2045-7758, 2045-7758\",\"shorttitle\":\"Fertilization modes and the evolution of sperm characteristics in marine fishes\",\"url\":\"https://onlinelibrary.wiley.com/doi/10.1002/ece3.9562\",\"doi\":\"10.1002/ece3.9562\",\"language\":\"en\",\"number\":\"12\",\"urldate\":\"2022-12-07\",\"journal\":\"Ecology and Evolution\",\"author\":[{\"propositions\":[],\"lastnames\":[\"Ito\"],\"firstnames\":[\"Takeshi\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Morita\"],\"firstnames\":[\"Masaya\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Okuno\"],\"firstnames\":[\"Seiya\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Inaba\"],\"firstnames\":[\"Kazuo\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Shiba\"],\"firstnames\":[\"Kogiku\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Munehara\"],\"firstnames\":[\"Hiroyuki\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Koya\"],\"firstnames\":[\"Yasunori\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Homma\"],\"firstnames\":[\"Mitsuo\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Awata\"],\"firstnames\":[\"Satoshi\"],\"suffixes\":[]}],\"month\":\"December\",\"year\":\"2022\",\"bibtex\":\"@article{ito_fertilization_2022,\\n\\ttitle = {Fertilization modes and the evolution of sperm characteristics in marine fishes: {Paired} comparisons of externally and internally fertilizing species},\\n\\tvolume = {12},\\n\\tissn = {2045-7758, 2045-7758},\\n\\tshorttitle = {Fertilization modes and the evolution of sperm characteristics in marine fishes},\\n\\turl = {https://onlinelibrary.wiley.com/doi/10.1002/ece3.9562},\\n\\tdoi = {10.1002/ece3.9562},\\n\\tlanguage = {en},\\n\\tnumber = {12},\\n\\turldate = {2022-12-07},\\n\\tjournal = {Ecology and Evolution},\\n\\tauthor = {Ito, Takeshi and Morita, Masaya and Okuno, Seiya and Inaba, Kazuo and Shiba, Kogiku and Munehara, Hiroyuki and Koya, Yasunori and Homma, Mitsuo and Awata, Satoshi},\\n\\tmonth = dec,\\n\\tyear = {2022},\\n}\\n\\n\",\"author_short\":[\"Ito, T.\",\"Morita, M.\",\"Okuno, S.\",\"Inaba, K.\",\"Shiba, K.\",\"Munehara, H.\",\"Koya, Y.\",\"Homma, M.\",\"Awata, S.\"],\"key\":\"ito_fertilization_2022\",\"id\":\"ito_fertilization_2022\",\"bibbaseid\":\"ito-morita-okuno-inaba-shiba-munehara-koya-homma-etal-fertilizationmodesandtheevolutionofspermcharacteristicsinmarinefishespairedcomparisonsofexternallyandinternallyfertilizingspecies-2022\",\"role\":\"author\",\"urls\":{\"Paper\":\"https://onlinelibrary.wiley.com/doi/10.1002/ece3.9562\"},\"metadata\":{\"authorlinks\":{\"inaba, k\":\"https://www.shimoda.tsukuba.ac.jp/en/staff_inaba.html\"}},\"html\":\"\"},{\"bibtype\":\"article\",\"type\":\"article\",\"title\":\"Two distinct compartments of a ctenophore comb plate provide structural and functional integrity for the motility of giant multicilia\",\"issn\":\"09609822\",\"url\":\"https://linkinghub.elsevier.com/retrieve/pii/S0960982222016037\",\"doi\":\"10.1016/j.cub.2022.09.061\",\"language\":\"en\",\"urldate\":\"2022-10-25\",\"journal\":\"Current Biology\",\"author\":[{\"propositions\":[],\"lastnames\":[\"Jokura\"],\"firstnames\":[\"Kei\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Sato\"],\"firstnames\":[\"Yu\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Shiba\"],\"firstnames\":[\"Kogiku\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Inaba\"],\"firstnames\":[\"Kazuo\"],\"suffixes\":[]}],\"month\":\"October\",\"year\":\"2022\",\"pages\":\"S0960982222016037\",\"bibtex\":\"@article{jokura_two_2022,\\n\\ttitle = {Two distinct compartments of a ctenophore comb plate provide structural and functional integrity for the motility of giant multicilia},\\n\\tissn = {09609822},\\n\\turl = {https://linkinghub.elsevier.com/retrieve/pii/S0960982222016037},\\n\\tdoi = {10.1016/j.cub.2022.09.061},\\n\\tlanguage = {en},\\n\\turldate = {2022-10-25},\\n\\tjournal = {Current Biology},\\n\\tauthor = {Jokura, Kei and Sato, Yu and Shiba, Kogiku and Inaba, Kazuo},\\n\\tmonth = oct,\\n\\tyear = {2022},\\n\\tpages = {S0960982222016037},\\n}\\n\\n\",\"author_short\":[\"Jokura, K.\",\"Sato, Y.\",\"Shiba, K.\",\"Inaba, K.\"],\"key\":\"jokura_two_2022\",\"id\":\"jokura_two_2022\",\"bibbaseid\":\"jokura-sato-shiba-inaba-twodistinctcompartmentsofactenophorecombplateprovidestructuralandfunctionalintegrityforthemotilityofgiantmulticilia-2022\",\"role\":\"author\",\"urls\":{\"Paper\":\"https://linkinghub.elsevier.com/retrieve/pii/S0960982222016037\"},\"metadata\":{\"authorlinks\":{\"inaba, k\":\"https://www.shimoda.tsukuba.ac.jp/en/staff_inaba.html\"}},\"html\":\"\"},{\"bibtype\":\"article\",\"type\":\"article\",\"title\":\"JAMBIO and its coastal organism joint surveys: Network of marine stations explores Japanese coastal biota\",\"volume\":\"39\",\"issn\":\"0289-0003\",\"shorttitle\":\"JAMBIO and Its Coastal Organism Joint Surveys\",\"url\":\"https://bioone.org/journals/zoological-science/volume-39/issue-1/zs210069/JAMBIO-and-Its-Coastal-Organism-Joint-Surveys--Network-of/10.2108/zs210069.full\",\"doi\":\"10.2108/zs210069\",\"number\":\"1\",\"urldate\":\"2022-02-04\",\"journal\":\"Zoological Science\",\"author\":[{\"propositions\":[],\"lastnames\":[\"Nakano\"],\"firstnames\":[\"Hiroaki\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Isowa\"],\"firstnames\":[\"Yukinobu\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Inaba\"],\"firstnames\":[\"Kazuo\"],\"suffixes\":[]}],\"month\":\"February\",\"year\":\"2022\",\"pages\":\"1–6\",\"bibtex\":\"@article{nakano_jambio_2022,\\n\\ttitle = {{JAMBIO} and its coastal organism joint surveys: {Network} of marine stations explores {Japanese} coastal biota},\\n\\tvolume = {39},\\n\\tissn = {0289-0003},\\n\\tshorttitle = {{JAMBIO} and {Its} {Coastal} {Organism} {Joint} {Surveys}},\\n\\turl = {https://bioone.org/journals/zoological-science/volume-39/issue-1/zs210069/JAMBIO-and-Its-Coastal-Organism-Joint-Surveys--Network-of/10.2108/zs210069.full},\\n\\tdoi = {10.2108/zs210069},\\n\\tnumber = {1},\\n\\turldate = {2022-02-04},\\n\\tjournal = {Zoological Science},\\n\\tauthor = {Nakano, Hiroaki and Isowa, Yukinobu and Inaba, Kazuo},\\n\\tmonth = feb,\\n\\tyear = {2022},\\n\\tpages = {1--6},\\n}\\n\\n\",\"author_short\":[\"Nakano, H.\",\"Isowa, Y.\",\"Inaba, K.\"],\"key\":\"nakano_jambio_2022\",\"id\":\"nakano_jambio_2022\",\"bibbaseid\":\"nakano-isowa-inaba-jambioanditscoastalorganismjointsurveysnetworkofmarinestationsexploresjapanesecoastalbiota-2022\",\"role\":\"author\",\"urls\":{\"Paper\":\"https://bioone.org/journals/zoological-science/volume-39/issue-1/zs210069/JAMBIO-and-Its-Coastal-Organism-Joint-Surveys--Network-of/10.2108/zs210069.full\"},\"metadata\":{\"authorlinks\":{\"inaba, k\":\"https://www.shimoda.tsukuba.ac.jp/en/staff_inaba.html\"}},\"downloads\":30,\"html\":\"\"},{\"bibtype\":\"article\",\"type\":\"article\",\"title\":\"The roles of two CNG channels in the regulation of ascidian sperm chemotaxis\",\"volume\":\"23\",\"issn\":\"1422-0067\",\"url\":\"https://www.mdpi.com/1422-0067/23/3/1648\",\"doi\":\"10.3390/ijms23031648\",\"abstract\":\"Spermatozoa sense and respond to their environmental signals to ensure fertilization success. Reception and transduction of signals are reflected rapidly in sperm flagellar waveforms and swimming behavior. In the ascidian Ciona intestinalis (type A; also called C. robusta), an egg-derived sulfated steroid called SAAF (sperm activating and attracting factor), induces both sperm motility activation and chemotaxis. Two types of CNG (cyclic nucleotide-gated) channels, Ci-tetra KCNG (tetrameric, cyclic nucleotide-gated, K+-selective) and Ci-HCN (hyperpolarization-activated and cyclic nucleotide-gated), are highly expressed in Ciona testis from the comprehensive gene expression analysis. To elucidate the sperm signaling pathway to regulate flagellar motility, we focus on the role of CNG channels. In this study, the immunochemical analysis revealed that both CNG channels are expressed in Ciona sperm and localized to sperm flagella. Sperm motility analysis and Ca2+ imaging during chemotaxis showed that CNG channel inhibition affected the changes in flagellar waveforms and Ca2+ efflux needed for the chemotactic turn. These results suggest that CNG channels in Ciona sperm play a vital role in regulating sperm motility and intracellular Ca2+ regulation during chemotaxis.\",\"language\":\"en\",\"number\":\"3\",\"urldate\":\"2022-02-07\",\"journal\":\"International Journal of Molecular Sciences\",\"author\":[{\"propositions\":[],\"lastnames\":[\"Shiba\"],\"firstnames\":[\"Kogiku\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Inaba\"],\"firstnames\":[\"Kazuo\"],\"suffixes\":[]}],\"month\":\"January\",\"year\":\"2022\",\"pages\":\"1648\",\"bibtex\":\"@article{shiba_roles_2022,\\n\\ttitle = {The roles of two {CNG} channels in the regulation of ascidian sperm chemotaxis},\\n\\tvolume = {23},\\n\\tissn = {1422-0067},\\n\\turl = {https://www.mdpi.com/1422-0067/23/3/1648},\\n\\tdoi = {10.3390/ijms23031648},\\n\\tabstract = {Spermatozoa sense and respond to their environmental signals to ensure fertilization success. Reception and transduction of signals are reflected rapidly in sperm flagellar waveforms and swimming behavior. In the ascidian Ciona intestinalis (type A; also called C. robusta), an egg-derived sulfated steroid called SAAF (sperm activating and attracting factor), induces both sperm motility activation and chemotaxis. Two types of CNG (cyclic nucleotide-gated) channels, Ci-tetra KCNG (tetrameric, cyclic nucleotide-gated, K+-selective) and Ci-HCN (hyperpolarization-activated and cyclic nucleotide-gated), are highly expressed in Ciona testis from the comprehensive gene expression analysis. To elucidate the sperm signaling pathway to regulate flagellar motility, we focus on the role of CNG channels. In this study, the immunochemical analysis revealed that both CNG channels are expressed in Ciona sperm and localized to sperm flagella. Sperm motility analysis and Ca2+ imaging during chemotaxis showed that CNG channel inhibition affected the changes in flagellar waveforms and Ca2+ efflux needed for the chemotactic turn. These results suggest that CNG channels in Ciona sperm play a vital role in regulating sperm motility and intracellular Ca2+ regulation during chemotaxis.},\\n\\tlanguage = {en},\\n\\tnumber = {3},\\n\\turldate = {2022-02-07},\\n\\tjournal = {International Journal of Molecular Sciences},\\n\\tauthor = {Shiba, Kogiku and Inaba, Kazuo},\\n\\tmonth = jan,\\n\\tyear = {2022},\\n\\tpages = {1648},\\n}\\n\\n\",\"author_short\":[\"Shiba, K.\",\"Inaba, K.\"],\"key\":\"shiba_roles_2022\",\"id\":\"shiba_roles_2022\",\"bibbaseid\":\"shiba-inaba-therolesoftwocngchannelsintheregulationofascidianspermchemotaxis-2022\",\"role\":\"author\",\"urls\":{\"Paper\":\"https://www.mdpi.com/1422-0067/23/3/1648\"},\"metadata\":{\"authorlinks\":{\"inaba, k\":\"https://www.shimoda.tsukuba.ac.jp/en/staff_inaba.html\"}},\"downloads\":18,\"html\":\"\"},{\"bibtype\":\"article\",\"type\":\"article\",\"title\":\"Axonemal growth and alignment during paraspermatogenesis in the marine gastropod <i>Strombus luhuanus</i>\",\"volume\":\"10\",\"issn\":\"2296-634X\",\"url\":\"https://www.frontiersin.org/articles/10.3389/fcell.2022.905748/full\",\"doi\":\"10.3389/fcell.2022.905748\",\"abstract\":\"Parasperm are non-fertilizing sperm that are produced simultaneously with fertile eusperm. They occur in several animal species and show considerable morphological diversity. We investigated the dynamics of axonemes during paraspermatogenesis in the marine snail S. luhuanus . Mature parasperm were characterized by two lateral undulating membranes for motility and many globular vesicles. Axonemes were first observed as brush-like structures that extruded from the anterior region. Multiple axonemes longer than the brush then started to extend inside the cytoplasm towards the posterior region. The mass of the axonemes separated into two lateral rows and formed an undulating membrane that drives bidirectional swimming in the mature parasperm. The central pair of axonemes was aligned in the undulating membrane, resulting in cooperative bend propagation. During paraspermatogenesis, centrioles were largely diminished and localized to the anterior region. CEP290, a major component of the transition zone, showed a broad distribution in the anterior area. Axonemes in the posterior region showed a 9 + 0 structure with both outer and inner arm dyneins. These observations provide a structural basis for understanding the physiological functions of parasperm in marine reproductive strategies.\",\"urldate\":\"2022-07-12\",\"journal\":\"Frontiers in Cell and Developmental Biology\",\"author\":[{\"propositions\":[],\"lastnames\":[\"Shibata\"],\"firstnames\":[\"Daisuke\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Morita\"],\"firstnames\":[\"Masaya\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Sato\"],\"firstnames\":[\"Yu\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Shiba\"],\"firstnames\":[\"Kogiku\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Kitanobo\"],\"firstnames\":[\"Seiya\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Yokoya\"],\"firstnames\":[\"Ryo\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Inaba\"],\"firstnames\":[\"Kazuo\"],\"suffixes\":[]}],\"month\":\"June\",\"year\":\"2022\",\"pages\":\"905748\",\"bibtex\":\"@article{shibata_axonemal_2022,\\n\\ttitle = {Axonemal growth and alignment during paraspermatogenesis in the marine gastropod \\\\textit{{Strombus} luhuanus}},\\n\\tvolume = {10},\\n\\tissn = {2296-634X},\\n\\turl = {https://www.frontiersin.org/articles/10.3389/fcell.2022.905748/full},\\n\\tdoi = {10.3389/fcell.2022.905748},\\n\\tabstract = {Parasperm are non-fertilizing sperm that are produced simultaneously with fertile eusperm. They occur in several animal species and show considerable morphological diversity. We investigated the dynamics of axonemes during paraspermatogenesis in the marine snail\\n              S. luhuanus\\n              . Mature parasperm were characterized by two lateral undulating membranes for motility and many globular vesicles. Axonemes were first observed as brush-like structures that extruded from the anterior region. Multiple axonemes longer than the brush then started to extend inside the cytoplasm towards the posterior region. The mass of the axonemes separated into two lateral rows and formed an undulating membrane that drives bidirectional swimming in the mature parasperm. The central pair of axonemes was aligned in the undulating membrane, resulting in cooperative bend propagation. During paraspermatogenesis, centrioles were largely diminished and localized to the anterior region. CEP290, a major component of the transition zone, showed a broad distribution in the anterior area. Axonemes in the posterior region showed a 9 + 0 structure with both outer and inner arm dyneins. These observations provide a structural basis for understanding the physiological functions of parasperm in marine reproductive strategies.},\\n\\turldate = {2022-07-12},\\n\\tjournal = {Frontiers in Cell and Developmental Biology},\\n\\tauthor = {Shibata, Daisuke and Morita, Masaya and Sato, Yu and Shiba, Kogiku and Kitanobo, Seiya and Yokoya, Ryo and Inaba, Kazuo},\\n\\tmonth = jun,\\n\\tyear = {2022},\\n\\tpages = {905748},\\n}\\n\\n\",\"author_short\":[\"Shibata, D.\",\"Morita, M.\",\"Sato, Y.\",\"Shiba, K.\",\"Kitanobo, S.\",\"Yokoya, R.\",\"Inaba, K.\"],\"key\":\"shibata_axonemal_2022\",\"id\":\"shibata_axonemal_2022\",\"bibbaseid\":\"shibata-morita-sato-shiba-kitanobo-yokoya-inaba-axonemalgrowthandalignmentduringparaspermatogenesisinthemarinegastropodistrombusluhuanusi-2022\",\"role\":\"author\",\"urls\":{\"Paper\":\"https://www.frontiersin.org/articles/10.3389/fcell.2022.905748/full\"},\"metadata\":{\"authorlinks\":{\"inaba, k\":\"https://www.shimoda.tsukuba.ac.jp/en/staff_inaba.html\"}},\"downloads\":18,\"html\":\"\"},{\"bibtype\":\"article\",\"type\":\"article\",\"title\":\"Morphological differences in tardigrade spermatozoa induce variation in gamete motility\",\"volume\":\"7\",\"issn\":\"2056-3132\",\"url\":\"https:///bmczool.biomedcentral.com/articles/10.1186/s40850-022-00109-w\",\"doi\":\"10.1186/s40850-022-00109-w\",\"abstract\":\"Abstract Background Fertilization is an event at the beginning of ontogeny. Successful fertilization depends on strategies for uniting female and male gametes that developed throughout evolutionary history. In some species of tardigrades, investigations of reproduction have revealed that released spermatozoa swim in the water to reach a female, after which the gametes are stored in her body. The morphology of the spermatozoa includes a coiled nucleus and a species-specific-length acrosome. Although the mating behaviour and morphology of tardigrades have been reported, the motility of male gametes remains unknown. Here, using a high-speed camera, we recorded the spermatozoon motilities of two tardigrades, Paramacrobiotus sp. and Macrobiotus shonaicus, which have longer and shorter spermatozoa, respectively. Results The movement of spermatozoa was faster in Paramacrobiotus sp. than in M. shonaicus , but the beat frequencies of the tails were equal, suggesting that the long tail improved acceleration. In both species, the head part consisting of a coiled nucleus and an acrosome did not swing, in contrast to the tail. The head part of Paramacrobiotus sp. spermatozoa swung harder during turning; in contrast, the tail of M. shonaicus moved more widely than the head. Finally, after mating, the spermatozoa that reached the female aggregated around the cloaca while waiting to enter her body in both tested species. Conclusions This study provides results for the first observations and analyses of individual spermatozoon motility in tardigrades. A comparison of the spermatozoon movements of the two tardigrades suggested that the motilities of the male gametes were affected by morphological differences, where the longer spermatozoa swam faster and the shorter ones showed more stable swimming. Swimming was mainly induced by tail movement, but the long head of Paramacrobiotus sp. spermatozoa might be especially important for turning. In addition, observations of mated female cloacae suggested that the head parts of the spermatozoa were required for aggregation around the cloaca of a mated female.\",\"language\":\"en\",\"number\":\"1\",\"urldate\":\"2022-02-07\",\"journal\":\"BMC Zoology\",\"author\":[{\"propositions\":[],\"lastnames\":[\"Sugiura\"],\"firstnames\":[\"Kenta\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Shiba\"],\"firstnames\":[\"Kogiku\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Inaba\"],\"firstnames\":[\"Kazuo\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Matsumoto\"],\"firstnames\":[\"Midori\"],\"suffixes\":[]}],\"month\":\"December\",\"year\":\"2022\",\"pages\":\"8\",\"bibtex\":\"@article{sugiura_morphological_2022,\\n\\ttitle = {Morphological differences in tardigrade spermatozoa induce variation in gamete motility},\\n\\tvolume = {7},\\n\\tissn = {2056-3132},\\n\\turl = {https:///bmczool.biomedcentral.com/articles/10.1186/s40850-022-00109-w},\\n\\tdoi = {10.1186/s40850-022-00109-w},\\n\\tabstract = {Abstract\\n            \\n              Background\\n              \\n                Fertilization is an event at the beginning of ontogeny. Successful fertilization depends on strategies for uniting female and male gametes that developed throughout evolutionary history. In some species of tardigrades, investigations of reproduction have revealed that released spermatozoa swim in the water to reach a female, after which the gametes are stored in her body. The morphology of the spermatozoa includes a coiled nucleus and a species-specific-length acrosome. Although the mating behaviour and morphology of tardigrades have been reported, the motility of male gametes remains unknown. Here, using a high-speed camera, we recorded the spermatozoon motilities of two tardigrades,\\n                Paramacrobiotus\\n                sp. and\\n                Macrobiotus shonaicus,\\n                which have longer and shorter spermatozoa, respectively.\\n              \\n            \\n            \\n              Results\\n              \\n                The movement of spermatozoa was faster in\\n                Paramacrobiotus\\n                sp. than in\\n                M. shonaicus\\n                , but the beat frequencies of the tails were equal, suggesting that the long tail improved acceleration. In both species, the head part consisting of a coiled nucleus and an acrosome did not swing, in contrast to the tail. The head part of\\n                Paramacrobiotus\\n                sp. spermatozoa swung harder during turning; in contrast, the tail of\\n                M. shonaicus\\n                moved more widely than the head. Finally, after mating, the spermatozoa that reached the female aggregated around the cloaca while waiting to enter her body in both tested species.\\n              \\n            \\n            \\n              Conclusions\\n              \\n                This study provides results for the first observations and analyses of individual spermatozoon motility in tardigrades. A comparison of the spermatozoon movements of the two tardigrades suggested that the motilities of the male gametes were affected by morphological differences, where the longer spermatozoa swam faster and the shorter ones showed more stable swimming. Swimming was mainly induced by tail movement, but the long head of\\n                Paramacrobiotus\\n                sp. spermatozoa might be especially important for turning. In addition, observations of mated female cloacae suggested that the head parts of the spermatozoa were required for aggregation around the cloaca of a mated female.},\\n\\tlanguage = {en},\\n\\tnumber = {1},\\n\\turldate = {2022-02-07},\\n\\tjournal = {BMC Zoology},\\n\\tauthor = {Sugiura, Kenta and Shiba, Kogiku and Inaba, Kazuo and Matsumoto, Midori},\\n\\tmonth = dec,\\n\\tyear = {2022},\\n\\tpages = {8},\\n}\\n\\n\",\"author_short\":[\"Sugiura, K.\",\"Shiba, K.\",\"Inaba, K.\",\"Matsumoto, M.\"],\"key\":\"sugiura_morphological_2022\",\"id\":\"sugiura_morphological_2022\",\"bibbaseid\":\"sugiura-shiba-inaba-matsumoto-morphologicaldifferencesintardigradespermatozoainducevariationingametemotility-2022\",\"role\":\"author\",\"urls\":{\"Paper\":\"https:///bmczool.biomedcentral.com/articles/10.1186/s40850-022-00109-w\"},\"metadata\":{\"authorlinks\":{\"inaba, k\":\"https://www.shimoda.tsukuba.ac.jp/en/staff_inaba.html\"}},\"downloads\":18,\"html\":\"\"}],\n      metadata: {\"owner\":\"inaba, k\",\"authorlinks\":{\"inaba, k\":\"https://www.shimoda.tsukuba.ac.jp/en/staff_inaba.html\"}},\n      ownerID: \"jgRCsGpuBBveArQQZ\"\n    };\n  </script>\n\n  <script type=\"text/javascript\">\n  var site$;\n  if (typeof $ != 'undefined') {\n    console.log('existing jquery: storing and then restoring');\n    site$ = $;\n    $ = null;\n  }\n  </script>\n\n  <script src=\"https://ajax.googleapis.com/ajax/libs/jquery/2.2.4/jquery.min.js\">\n  </script>\n  <script type=\"text/javascript\">\n  if (site$) {\n    console.log('existing jquery: avoiding conflict and restoring');\n    bb$ = $.noConflict(true);\n    $ = site$;\n  } else {\n    bb$ = $;\n  }\n  </script>\n\n  <script src=\"//bibbase.org/js/bibbase.min.js\"\n          type=\"text/javascript\">\n  </script>\n\n  <script type=\"text/javascript\"\n          src=\"https://cdnjs.cloudflare.com/ajax/libs/mathjax/2.7.1/MathJax.js?config=TeX-AMS-MML_HTMLorMML\">\n  </script>\n  <script type=\"text/x-mathjax-config\">\n    MathJax.Hub.Config({tex2jax: {inlineMath: [['$','$'], ['\\\\(','\\\\)']]},\n                        skipTags: [\"body\"],\n                        processClass: \"bibbase_paper\"});\n  </script>\n\n  <style>\n  @media print {\n    .dontprint {\n        display: none !important;\n    }\n\n  .bibbase_group {\n    background-color: #E0E0E0;\n    font-style: italic;\n    font-size: larger;\n    text-shadow: 0px 0px 3px #FFFFFF;\n    border-radius: 4px 4px 4px 4px;\n    -moz-border-radius: 4px 4px 4px 4px;\n    -webkit-border-radius: 4px;\n    padding: 5px 40px 5px;\n    margin-top: 10px;\n    margin-bottom: 5px;\n    cursor: pointer;\n  }\n\n  .bibbase_paper {\n    margin-bottom: 5px;\n  }\n\n  }\n  </style>\n\n  \n  <div style=\"float: right; margin-right: 10px; margin-top: 10px; text-align: right; font-size: 12px\">\n    generated by\n    <a href=\"//bibbase.org\"\n       onclick=\"trackOutboundLink('bibbase', 'logo clicked', window.location.href, '//bibbase.org'); return false;\">\n      <img src=\"//bibbase.org/img/logo.svg\"\n           style=\"vertical-align: middle; margin-left: 3px; height: 16px;\"/\n           alt=\"bibbase.org\"\n           title=\"BibBase – The easiest way to maintain your publications page.\"\n        ></a>\n    \n  </div>\n  \n\n  <div id=\"bibbase_header\" class=\"dontprint\" style=\"display: none\">\n\n    <ul class=\"nav nav-pills\" style=\"margin: 0px;\">\n\n      <li class=\"dropdown\" id=\"groupby_dropdown\">\n      <a class=\"dropdown-toggle\"\n         data-toggle=\"dropdown\"\n         href=\"#\">\n          Group by\n          <b class=\"caret\"></b>\n      </a>\n      <ul class=\"dropdown-menu\">\n        <li class=\"groupby year\"><a onclick=\"groupby('year')\">\n            Year</a>\n        </li>\n        <li class=\"groupby author\"><a onclick=\"groupby('author_short')\">\n            Author</a>\n        </li>\n        <li class=\"groupby type\"><a onclick=\"groupby('type')\">\n            Type</a>\n        </li>\n        <li class=\"groupby keyword\"><a onclick=\"groupby('keyword')\">\n            Keyword</a>\n        </li>\n        <li class=\"groupby downloads\"><a onclick=\"groupby('downloads')\">\n            Downloads</a>\n        </li>\n      </ul>\n      </li>\n\n\n      <li class=\"dropdown\" id=\"menu_dropdown\">\n      <a class=\"dropdown-toggle\"\n         data-toggle=\"dropdown\"\n         href=\"#\" alt=\"controls\">\n          <i class=\"fa fa-reorder\" alt=\"controls\"></i>\n          <b class=\"caret\"></b>\n      </a>\n      <ul class=\"dropdown-menu\">\n        <li>\n          <a onclick=\"toggleAll()\">\n            <i class=\"fa fa-chevron-down fa-fw\"></i> Expand/Collapse All\n          </a>\n        </li>\n        <li>\n          <a download=\"items\" href=\"data:text/bibtex;base64,@article{asai_new_2022,
	title = {A new species of {Acoela} possessing a middorsal appendage with a possible sensory function},
	volume = {39},
	issn = {0289-0003},
	url = {https://bioone.org/journals/zoological-science/volume-39/issue-1/zs210058/A-New-Species-of-Acoela-Possessing-a-Middorsal-Appendage-with/10.2108/zs210058.full},
	doi = {10.2108/zs210058},
	number = {1},
	urldate = {2022-01-25},
	journal = {Zoological Science},
	author = {Asai, Masashi and Miyazawa, Hideyuki and Yanase, Ryuji and Inaba, Kazuo and Nakano, Hiroaki},
	month = jan,
	year = {2022},
	pages = {147--156},
}



@article{hayakawa_mass_2022,
	title = {Mass spectrometry of short peptides reveals common features of metazoan peptidergic neurons},
	volume = {6},
	issn = {2397-334X},
	url = {https://www.nature.com/articles/s41559-022-01835-7},
	doi = {10.1038/s41559-022-01835-7},
	abstract = {Abstract
            The evolutionary origins of neurons remain unknown. Although recent genome data of extant early-branching animals have shown that neural genes existed in the common ancestor of animals, the physiological and genetic properties of neurons in the early evolutionary phase are still unclear. Here, we performed a mass spectrometry-based comprehensive survey of short peptides from early-branching lineages Cnidaria, Porifera and Ctenophora. We identified a number of mature ctenophore neuropeptides that are expressed in neurons associated with sensory, muscular and digestive systems. The ctenophore peptides are stored in vesicles in cell bodies and neurites, suggesting volume transmission similar to that of cnidarian and bilaterian peptidergic systems. A comparison of genetic characteristics revealed that the peptide-expressing cells of Cnidaria and Ctenophora express the vast majority of genes that have pivotal roles in maturation, secretion and degradation of neuropeptides in Bilateria. Functional analysis of neuropeptides and prediction of receptors with machine learning demonstrated peptide regulation of a wide range of target effector cells, including cells of muscular systems. The striking parallels between the peptidergic neuronal properties of Cnidaria and Bilateria and those of Ctenophora, the most basal neuron-bearing animals, suggest a common evolutionary origin of metazoan peptidergic nervous systems.},
	language = {en},
	number = {10},
	urldate = {2022-10-25},
	journal = {Nature Ecology \& Evolution},
	author = {Hayakawa, Eisuke and Guzman, Christine and Horiguchi, Osamu and Kawano, Chihiro and Shiraishi, Akira and Mohri, Kurato and Lin, Mei-Fang and Nakamura, Ryotaro and Nakamura, Ryo and Kawai, Erina and Komoto, Shinya and Jokura, Kei and Shiba, Kogiku and Shigenobu, Shuji and Satake, Honoo and Inaba, Kazuo and Watanabe, Hiroshi},
	month = aug,
	year = {2022},
	pages = {1438--1448},
}



@article{ito_fertilization_2022,
	title = {Fertilization modes and the evolution of sperm characteristics in marine fishes: {Paired} comparisons of externally and internally fertilizing species},
	volume = {12},
	issn = {2045-7758, 2045-7758},
	shorttitle = {Fertilization modes and the evolution of sperm characteristics in marine fishes},
	url = {https://onlinelibrary.wiley.com/doi/10.1002/ece3.9562},
	doi = {10.1002/ece3.9562},
	language = {en},
	number = {12},
	urldate = {2022-12-07},
	journal = {Ecology and Evolution},
	author = {Ito, Takeshi and Morita, Masaya and Okuno, Seiya and Inaba, Kazuo and Shiba, Kogiku and Munehara, Hiroyuki and Koya, Yasunori and Homma, Mitsuo and Awata, Satoshi},
	month = dec,
	year = {2022},
}



@article{jokura_two_2022,
	title = {Two distinct compartments of a ctenophore comb plate provide structural and functional integrity for the motility of giant multicilia},
	issn = {09609822},
	url = {https://linkinghub.elsevier.com/retrieve/pii/S0960982222016037},
	doi = {10.1016/j.cub.2022.09.061},
	language = {en},
	urldate = {2022-10-25},
	journal = {Current Biology},
	author = {Jokura, Kei and Sato, Yu and Shiba, Kogiku and Inaba, Kazuo},
	month = oct,
	year = {2022},
	pages = {S0960982222016037},
}



@article{nakano_jambio_2022,
	title = {{JAMBIO} and its coastal organism joint surveys: {Network} of marine stations explores {Japanese} coastal biota},
	volume = {39},
	issn = {0289-0003},
	shorttitle = {{JAMBIO} and {Its} {Coastal} {Organism} {Joint} {Surveys}},
	url = {https://bioone.org/journals/zoological-science/volume-39/issue-1/zs210069/JAMBIO-and-Its-Coastal-Organism-Joint-Surveys--Network-of/10.2108/zs210069.full},
	doi = {10.2108/zs210069},
	number = {1},
	urldate = {2022-02-04},
	journal = {Zoological Science},
	author = {Nakano, Hiroaki and Isowa, Yukinobu and Inaba, Kazuo},
	month = feb,
	year = {2022},
	pages = {1--6},
}



@article{shiba_roles_2022,
	title = {The roles of two {CNG} channels in the regulation of ascidian sperm chemotaxis},
	volume = {23},
	issn = {1422-0067},
	url = {https://www.mdpi.com/1422-0067/23/3/1648},
	doi = {10.3390/ijms23031648},
	abstract = {Spermatozoa sense and respond to their environmental signals to ensure fertilization success. Reception and transduction of signals are reflected rapidly in sperm flagellar waveforms and swimming behavior. In the ascidian Ciona intestinalis (type A; also called C. robusta), an egg-derived sulfated steroid called SAAF (sperm activating and attracting factor), induces both sperm motility activation and chemotaxis. Two types of CNG (cyclic nucleotide-gated) channels, Ci-tetra KCNG (tetrameric, cyclic nucleotide-gated, K+-selective) and Ci-HCN (hyperpolarization-activated and cyclic nucleotide-gated), are highly expressed in Ciona testis from the comprehensive gene expression analysis. To elucidate the sperm signaling pathway to regulate flagellar motility, we focus on the role of CNG channels. In this study, the immunochemical analysis revealed that both CNG channels are expressed in Ciona sperm and localized to sperm flagella. Sperm motility analysis and Ca2+ imaging during chemotaxis showed that CNG channel inhibition affected the changes in flagellar waveforms and Ca2+ efflux needed for the chemotactic turn. These results suggest that CNG channels in Ciona sperm play a vital role in regulating sperm motility and intracellular Ca2+ regulation during chemotaxis.},
	language = {en},
	number = {3},
	urldate = {2022-02-07},
	journal = {International Journal of Molecular Sciences},
	author = {Shiba, Kogiku and Inaba, Kazuo},
	month = jan,
	year = {2022},
	pages = {1648},
}



@article{shibata_axonemal_2022,
	title = {Axonemal growth and alignment during paraspermatogenesis in the marine gastropod \textit{{Strombus} luhuanus}},
	volume = {10},
	issn = {2296-634X},
	url = {https://www.frontiersin.org/articles/10.3389/fcell.2022.905748/full},
	doi = {10.3389/fcell.2022.905748},
	abstract = {Parasperm are non-fertilizing sperm that are produced simultaneously with fertile eusperm. They occur in several animal species and show considerable morphological diversity. We investigated the dynamics of axonemes during paraspermatogenesis in the marine snail
              S. luhuanus
              . Mature parasperm were characterized by two lateral undulating membranes for motility and many globular vesicles. Axonemes were first observed as brush-like structures that extruded from the anterior region. Multiple axonemes longer than the brush then started to extend inside the cytoplasm towards the posterior region. The mass of the axonemes separated into two lateral rows and formed an undulating membrane that drives bidirectional swimming in the mature parasperm. The central pair of axonemes was aligned in the undulating membrane, resulting in cooperative bend propagation. During paraspermatogenesis, centrioles were largely diminished and localized to the anterior region. CEP290, a major component of the transition zone, showed a broad distribution in the anterior area. Axonemes in the posterior region showed a 9 + 0 structure with both outer and inner arm dyneins. These observations provide a structural basis for understanding the physiological functions of parasperm in marine reproductive strategies.},
	urldate = {2022-07-12},
	journal = {Frontiers in Cell and Developmental Biology},
	author = {Shibata, Daisuke and Morita, Masaya and Sato, Yu and Shiba, Kogiku and Kitanobo, Seiya and Yokoya, Ryo and Inaba, Kazuo},
	month = jun,
	year = {2022},
	pages = {905748},
}



@article{sugiura_morphological_2022,
	title = {Morphological differences in tardigrade spermatozoa induce variation in gamete motility},
	volume = {7},
	issn = {2056-3132},
	url = {https:///bmczool.biomedcentral.com/articles/10.1186/s40850-022-00109-w},
	doi = {10.1186/s40850-022-00109-w},
	abstract = {Abstract
            
              Background
              
                Fertilization is an event at the beginning of ontogeny. Successful fertilization depends on strategies for uniting female and male gametes that developed throughout evolutionary history. In some species of tardigrades, investigations of reproduction have revealed that released spermatozoa swim in the water to reach a female, after which the gametes are stored in her body. The morphology of the spermatozoa includes a coiled nucleus and a species-specific-length acrosome. Although the mating behaviour and morphology of tardigrades have been reported, the motility of male gametes remains unknown. Here, using a high-speed camera, we recorded the spermatozoon motilities of two tardigrades,
                Paramacrobiotus
                sp. and
                Macrobiotus shonaicus,
                which have longer and shorter spermatozoa, respectively.
              
            
            
              Results
              
                The movement of spermatozoa was faster in
                Paramacrobiotus
                sp. than in
                M. shonaicus
                , but the beat frequencies of the tails were equal, suggesting that the long tail improved acceleration. In both species, the head part consisting of a coiled nucleus and an acrosome did not swing, in contrast to the tail. The head part of
                Paramacrobiotus
                sp. spermatozoa swung harder during turning; in contrast, the tail of
                M. shonaicus
                moved more widely than the head. Finally, after mating, the spermatozoa that reached the female aggregated around the cloaca while waiting to enter her body in both tested species.
              
            
            
              Conclusions
              
                This study provides results for the first observations and analyses of individual spermatozoon motility in tardigrades. A comparison of the spermatozoon movements of the two tardigrades suggested that the motilities of the male gametes were affected by morphological differences, where the longer spermatozoa swam faster and the shorter ones showed more stable swimming. Swimming was mainly induced by tail movement, but the long head of
                Paramacrobiotus
                sp. spermatozoa might be especially important for turning. In addition, observations of mated female cloacae suggested that the head parts of the spermatozoa were required for aggregation around the cloaca of a mated female.},
	language = {en},
	number = {1},
	urldate = {2022-02-07},
	journal = {BMC Zoology},
	author = {Sugiura, Kenta and Shiba, Kogiku and Inaba, Kazuo and Matsumoto, Midori},
	month = dec,
	year = {2022},
	pages = {8},
}

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In order to keep using Mendeley with BibBase past April\n        14th, you need to:\n        <ol>\n          <li>renew the authorization for BibBase on Mendeley, and</li>\n          <li>update the BibBase URL\n            in your page the same way you did when you initially set up\n            this page.\n          </li>\n        </ol>\n      </p>\n\n      <p>\n        <a href=\"http://bibbase.org/cgi-bin/mendeley2/get.py\">\n          <i class=\"fa fa-angle-double-right\"></i>\n          Fix it now</a>\n      </p>\n    </div>\n\n  </div>\n\n\n  <div id=\"bibbase_body\">\n    \n  \n  <div class=\"bibbase_paper\" id=\"asai-miyazawa-yanase-inaba-nakano-anewspeciesofacoelapossessingamiddorsalappendagewithapossiblesensoryfunction-2022\">\n  <span class=\"bibbase_paper_titleauthoryear\">\n\n  \n  <span class=\"bibbase_paper_author\">\n  Asai, M., Miyazawa, H., Yanase, R., <a class=\"bibbase author link\" href=\"https://www.shimoda.tsukuba.ac.jp/en/staff_inaba.html\">Inaba, K.</a>,  &amp; Nakano, H.\n</span>\n\n  <span class=\"bibbase_paper_title\">\n  \n  \n  \n  <a href=\"https://bioone.org/journals/zoological-science/volume-39/issue-1/zs210058/A-New-Species-of-Acoela-Possessing-a-Middorsal-Appendage-with/10.2108/zs210058.full\"\n     onclick=\"return trackOutboundLink('publications', 'click', 'asai-miyazawa-yanase-inaba-nakano-anewspeciesofacoelapossessingamiddorsalappendagewithapossiblesensoryfunction-2022', 'https://bioone.org/journals/zoological-science/volume-39/issue-1/zs210058/A-New-Species-of-Acoela-Possessing-a-Middorsal-Appendage-with/10.2108/zs210058.full', event);\">\n    A new species of Acoela possessing a middorsal appendage with a possible sensory function.\n  </a>\n  \n  \n  \n</span>\n\n  \n\n</span>\n\n  <i>Zoological Science</i>, 39(1): 147–156. January 2022.\n  <span class=\"note\"></span>\n\n<span class=\"bibbase_note\"></span>\n\n<br class=\"bibbase_paper_content\"/>\n\n<span class=\"bibbase_paper_content dontprint\">\n\n  \n  <a href=\"https://bioone.org/journals/zoological-science/volume-39/issue-1/zs210058/A-New-Species-of-Acoela-Possessing-a-Middorsal-Appendage-with/10.2108/zs210058.full\"\n     onclick=\"log_download('asai-miyazawa-yanase-inaba-nakano-anewspeciesofacoelapossessingamiddorsalappendagewithapossiblesensoryfunction-2022', 'https://bioone.org/journals/zoological-science/volume-39/issue-1/zs210058/A-New-Species-of-Acoela-Possessing-a-Middorsal-Appendage-with/10.2108/zs210058.full', event.ctrlKey, event)\">\n    <img src=\"//bibbase.org/img/filetypes/link.svg\"\n\t     alt=\"A new species of Acoela possessing a middorsal appendage with a possible sensory function [link]\"\n       title=\"Paper\"\n\t     class=\"bibbase_icon\"\n         ><span class=\"bibbase_icon_text\">Paper</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"http://doi.org/10.2108/zs210058\"\n     onclick=\"log_download('asai-miyazawa-yanase-inaba-nakano-anewspeciesofacoelapossessingamiddorsalappendagewithapossiblesensoryfunction-2022', 'http://doi.org/10.2108/zs210058', event.ctrlKey)\"\n     class=\"bibbase doi link\">\n    <span>doi</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"//bibbase.org/network/publication/asai-miyazawa-yanase-inaba-nakano-anewspeciesofacoelapossessingamiddorsalappendagewithapossiblesensoryfunction-2022\"\n     class=\"bibbase bibbase_page link\">link</a>\n  &nbsp;\n  \n\n  <a href=\"#\"\n     onclick=\"showBib(event); return false;\"\n     class=\"bibbase bibtex link\">bibtex\n    <i class=\"fa fa-caret-down\"></i></a>\n\n  \n\n  \n  &nbsp;\n  <span class=\"bibbase_stats_paper\" style=\"color: #777;\">\n    <span>36 downloads</span>\n  </span>\n  \n\n  <!-- <a class=\"bibbase read link hidden\" -->\n  <!--    href=\"javascript:toggleRead('asai-miyazawa-yanase-inaba-nakano-anewspeciesofacoelapossessingamiddorsalappendagewithapossiblesensoryfunction-2022')\" -->\n  <!--    title=\"Marking this paper as read adds it to your library on BibBase.\" -->\n  <!--    > -->\n  <!--   <i class=\"fa fa-check\"></i>&nbsp; -->\n  <!--   <span>mark as read</span> -->\n  <!-- </a> -->\n\n  &nbsp;\n  \n  \n\n</span>\n\n<div class=\"well well-small bibbase\" data-type=\"bibtex\"\n     style=\"display:none\">\n  <pre style=\"white-space: pre-wrap;\">@article{asai_new_2022,\n\ttitle = {A new species of {Acoela} possessing a middorsal appendage with a possible sensory function},\n\tvolume = {39},\n\tissn = {0289-0003},\n\turl = {https://bioone.org/journals/zoological-science/volume-39/issue-1/zs210058/A-New-Species-of-Acoela-Possessing-a-Middorsal-Appendage-with/10.2108/zs210058.full},\n\tdoi = {10.2108/zs210058},\n\tnumber = {1},\n\turldate = {2022-01-25},\n\tjournal = {Zoological Science},\n\tauthor = {Asai, Masashi and Miyazawa, Hideyuki and Yanase, Ryuji and Inaba, Kazuo and Nakano, Hiroaki},\n\tmonth = jan,\n\tyear = {2022},\n\tpages = {147--156},\n}\n\n</pre>\n</div>\n\n\n\n</div>\n\n\n  <div class=\"bibbase_paper\" id=\"hayakawa-guzman-horiguchi-kawano-shiraishi-mohri-lin-nakamura-etal-massspectrometryofshortpeptidesrevealscommonfeaturesofmetazoanpeptidergicneurons-2022\">\n  <span class=\"bibbase_paper_titleauthoryear\">\n\n  \n  <span class=\"bibbase_paper_author\">\n  Hayakawa, E., Guzman, C., Horiguchi, O., Kawano, C., Shiraishi, A., Mohri, K., Lin, M., Nakamura, R., Nakamura, R., Kawai, E., Komoto, S., Jokura, K., Shiba, K., Shigenobu, S., Satake, H., <a class=\"bibbase author link\" href=\"https://www.shimoda.tsukuba.ac.jp/en/staff_inaba.html\">Inaba, K.</a>,  &amp; Watanabe, H.\n</span>\n\n  <span class=\"bibbase_paper_title\">\n  \n  \n  \n  <a href=\"https://www.nature.com/articles/s41559-022-01835-7\"\n     onclick=\"return trackOutboundLink('publications', 'click', 'hayakawa-guzman-horiguchi-kawano-shiraishi-mohri-lin-nakamura-etal-massspectrometryofshortpeptidesrevealscommonfeaturesofmetazoanpeptidergicneurons-2022', 'https://www.nature.com/articles/s41559-022-01835-7', event);\">\n    Mass spectrometry of short peptides reveals common features of metazoan peptidergic neurons.\n  </a>\n  \n  \n  \n</span>\n\n  \n\n</span>\n\n  <i>Nature Ecology & Evolution</i>, 6(10): 1438–1448. August 2022.\n  <span class=\"note\"></span>\n\n<span class=\"bibbase_note\"></span>\n\n<br class=\"bibbase_paper_content\"/>\n\n<span class=\"bibbase_paper_content dontprint\">\n\n  \n  <a href=\"https://www.nature.com/articles/s41559-022-01835-7\"\n     onclick=\"log_download('hayakawa-guzman-horiguchi-kawano-shiraishi-mohri-lin-nakamura-etal-massspectrometryofshortpeptidesrevealscommonfeaturesofmetazoanpeptidergicneurons-2022', 'https://www.nature.com/articles/s41559-022-01835-7', event.ctrlKey, event)\">\n    <img src=\"//bibbase.org/img/filetypes/link.svg\"\n\t     alt=\"Mass spectrometry of short peptides reveals common features of metazoan peptidergic neurons [link]\"\n       title=\"Paper\"\n\t     class=\"bibbase_icon\"\n         ><span class=\"bibbase_icon_text\">Paper</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"http://doi.org/10.1038/s41559-022-01835-7\"\n     onclick=\"log_download('hayakawa-guzman-horiguchi-kawano-shiraishi-mohri-lin-nakamura-etal-massspectrometryofshortpeptidesrevealscommonfeaturesofmetazoanpeptidergicneurons-2022', 'http://doi.org/10.1038/s41559-022-01835-7', event.ctrlKey)\"\n     class=\"bibbase doi link\">\n    <span>doi</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"//bibbase.org/network/publication/hayakawa-guzman-horiguchi-kawano-shiraishi-mohri-lin-nakamura-etal-massspectrometryofshortpeptidesrevealscommonfeaturesofmetazoanpeptidergicneurons-2022\"\n     class=\"bibbase bibbase_page link\">link</a>\n  &nbsp;\n  \n\n  <a href=\"#\"\n     onclick=\"showBib(event); return false;\"\n     class=\"bibbase bibtex link\">bibtex\n    <i class=\"fa fa-caret-down\"></i></a>\n\n  \n  &nbsp;\n  <a class=\"bibbase_abstract_link bibbase link\"\n     href=\"#\"\n     onclick=\"showAbstract(event); return false;\">\n    abstract <i class=\"fa fa-caret-down\"></i></a>\n  \n\n  \n\n  <!-- <a class=\"bibbase read link hidden\" -->\n  <!--    href=\"javascript:toggleRead('hayakawa-guzman-horiguchi-kawano-shiraishi-mohri-lin-nakamura-etal-massspectrometryofshortpeptidesrevealscommonfeaturesofmetazoanpeptidergicneurons-2022')\" -->\n  <!--    title=\"Marking this paper as read adds it to your library on BibBase.\" -->\n  <!--    > -->\n  <!--   <i class=\"fa fa-check\"></i>&nbsp; -->\n  <!--   <span>mark as read</span> -->\n  <!-- </a> -->\n\n  &nbsp;\n  \n  \n\n</span>\n\n<div class=\"well well-small bibbase\" data-type=\"bibtex\"\n     style=\"display:none\">\n  <pre style=\"white-space: pre-wrap;\">@article{hayakawa_mass_2022,\n\ttitle = {Mass spectrometry of short peptides reveals common features of metazoan peptidergic neurons},\n\tvolume = {6},\n\tissn = {2397-334X},\n\turl = {https://www.nature.com/articles/s41559-022-01835-7},\n\tdoi = {10.1038/s41559-022-01835-7},\n\tabstract = {Abstract\n            The evolutionary origins of neurons remain unknown. Although recent genome data of extant early-branching animals have shown that neural genes existed in the common ancestor of animals, the physiological and genetic properties of neurons in the early evolutionary phase are still unclear. Here, we performed a mass spectrometry-based comprehensive survey of short peptides from early-branching lineages Cnidaria, Porifera and Ctenophora. We identified a number of mature ctenophore neuropeptides that are expressed in neurons associated with sensory, muscular and digestive systems. The ctenophore peptides are stored in vesicles in cell bodies and neurites, suggesting volume transmission similar to that of cnidarian and bilaterian peptidergic systems. A comparison of genetic characteristics revealed that the peptide-expressing cells of Cnidaria and Ctenophora express the vast majority of genes that have pivotal roles in maturation, secretion and degradation of neuropeptides in Bilateria. Functional analysis of neuropeptides and prediction of receptors with machine learning demonstrated peptide regulation of a wide range of target effector cells, including cells of muscular systems. The striking parallels between the peptidergic neuronal properties of Cnidaria and Bilateria and those of Ctenophora, the most basal neuron-bearing animals, suggest a common evolutionary origin of metazoan peptidergic nervous systems.},\n\tlanguage = {en},\n\tnumber = {10},\n\turldate = {2022-10-25},\n\tjournal = {Nature Ecology \\&amp; Evolution},\n\tauthor = {Hayakawa, Eisuke and Guzman, Christine and Horiguchi, Osamu and Kawano, Chihiro and Shiraishi, Akira and Mohri, Kurato and Lin, Mei-Fang and Nakamura, Ryotaro and Nakamura, Ryo and Kawai, Erina and Komoto, Shinya and Jokura, Kei and Shiba, Kogiku and Shigenobu, Shuji and Satake, Honoo and Inaba, Kazuo and Watanabe, Hiroshi},\n\tmonth = aug,\n\tyear = {2022},\n\tpages = {1438--1448},\n}\n\n</pre>\n</div>\n\n\n<div class=\"well well-small bibbase\" data-type=\"abstract\"\n     style=\"display:none\">\n  Abstract The evolutionary origins of neurons remain unknown. Although recent genome data of extant early-branching animals have shown that neural genes existed in the common ancestor of animals, the physiological and genetic properties of neurons in the early evolutionary phase are still unclear. Here, we performed a mass spectrometry-based comprehensive survey of short peptides from early-branching lineages Cnidaria, Porifera and Ctenophora. We identified a number of mature ctenophore neuropeptides that are expressed in neurons associated with sensory, muscular and digestive systems. The ctenophore peptides are stored in vesicles in cell bodies and neurites, suggesting volume transmission similar to that of cnidarian and bilaterian peptidergic systems. A comparison of genetic characteristics revealed that the peptide-expressing cells of Cnidaria and Ctenophora express the vast majority of genes that have pivotal roles in maturation, secretion and degradation of neuropeptides in Bilateria. Functional analysis of neuropeptides and prediction of receptors with machine learning demonstrated peptide regulation of a wide range of target effector cells, including cells of muscular systems. The striking parallels between the peptidergic neuronal properties of Cnidaria and Bilateria and those of Ctenophora, the most basal neuron-bearing animals, suggest a common evolutionary origin of metazoan peptidergic nervous systems.\n</div>\n\n\n</div>\n\n\n  <div class=\"bibbase_paper\" id=\"ito-morita-okuno-inaba-shiba-munehara-koya-homma-etal-fertilizationmodesandtheevolutionofspermcharacteristicsinmarinefishespairedcomparisonsofexternallyandinternallyfertilizingspecies-2022\">\n  <span class=\"bibbase_paper_titleauthoryear\">\n\n  \n  <span class=\"bibbase_paper_author\">\n  Ito, T., Morita, M., Okuno, S., <a class=\"bibbase author link\" href=\"https://www.shimoda.tsukuba.ac.jp/en/staff_inaba.html\">Inaba, K.</a>, Shiba, K., Munehara, H., Koya, Y., Homma, M.,  &amp; Awata, S.\n</span>\n\n  <span class=\"bibbase_paper_title\">\n  \n  \n  \n  <a href=\"https://onlinelibrary.wiley.com/doi/10.1002/ece3.9562\"\n     onclick=\"return trackOutboundLink('publications', 'click', 'ito-morita-okuno-inaba-shiba-munehara-koya-homma-etal-fertilizationmodesandtheevolutionofspermcharacteristicsinmarinefishespairedcomparisonsofexternallyandinternallyfertilizingspecies-2022', 'https://onlinelibrary.wiley.com/doi/10.1002/ece3.9562', event);\">\n    Fertilization modes and the evolution of sperm characteristics in marine fishes: Paired comparisons of externally and internally fertilizing species.\n  </a>\n  \n  \n  \n</span>\n\n  \n\n</span>\n\n  <i>Ecology and Evolution</i>, 12(12). December 2022.\n  <span class=\"note\"></span>\n\n<span class=\"bibbase_note\"></span>\n\n<br class=\"bibbase_paper_content\"/>\n\n<span class=\"bibbase_paper_content dontprint\">\n\n  \n  <a href=\"https://onlinelibrary.wiley.com/doi/10.1002/ece3.9562\"\n     onclick=\"log_download('ito-morita-okuno-inaba-shiba-munehara-koya-homma-etal-fertilizationmodesandtheevolutionofspermcharacteristicsinmarinefishespairedcomparisonsofexternallyandinternallyfertilizingspecies-2022', 'https://onlinelibrary.wiley.com/doi/10.1002/ece3.9562', event.ctrlKey, event)\">\n    <img src=\"//bibbase.org/img/filetypes/link.svg\"\n\t     alt=\"Fertilization modes and the evolution of sperm characteristics in marine fishes: Paired comparisons of externally and internally fertilizing species [link]\"\n       title=\"Paper\"\n\t     class=\"bibbase_icon\"\n         ><span class=\"bibbase_icon_text\">Paper</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"http://doi.org/10.1002/ece3.9562\"\n     onclick=\"log_download('ito-morita-okuno-inaba-shiba-munehara-koya-homma-etal-fertilizationmodesandtheevolutionofspermcharacteristicsinmarinefishespairedcomparisonsofexternallyandinternallyfertilizingspecies-2022', 'http://doi.org/10.1002/ece3.9562', event.ctrlKey)\"\n     class=\"bibbase doi link\">\n    <span>doi</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"//bibbase.org/network/publication/ito-morita-okuno-inaba-shiba-munehara-koya-homma-etal-fertilizationmodesandtheevolutionofspermcharacteristicsinmarinefishespairedcomparisonsofexternallyandinternallyfertilizingspecies-2022\"\n     class=\"bibbase bibbase_page link\">link</a>\n  &nbsp;\n  \n\n  <a href=\"#\"\n     onclick=\"showBib(event); return false;\"\n     class=\"bibbase bibtex link\">bibtex\n    <i class=\"fa fa-caret-down\"></i></a>\n\n  \n\n  \n\n  <!-- <a class=\"bibbase read link hidden\" -->\n  <!--    href=\"javascript:toggleRead('ito-morita-okuno-inaba-shiba-munehara-koya-homma-etal-fertilizationmodesandtheevolutionofspermcharacteristicsinmarinefishespairedcomparisonsofexternallyandinternallyfertilizingspecies-2022')\" -->\n  <!--    title=\"Marking this paper as read adds it to your library on BibBase.\" -->\n  <!--    > -->\n  <!--   <i class=\"fa fa-check\"></i>&nbsp; -->\n  <!--   <span>mark as read</span> -->\n  <!-- </a> -->\n\n  &nbsp;\n  \n  \n\n</span>\n\n<div class=\"well well-small bibbase\" data-type=\"bibtex\"\n     style=\"display:none\">\n  <pre style=\"white-space: pre-wrap;\">@article{ito_fertilization_2022,\n\ttitle = {Fertilization modes and the evolution of sperm characteristics in marine fishes: {Paired} comparisons of externally and internally fertilizing species},\n\tvolume = {12},\n\tissn = {2045-7758, 2045-7758},\n\tshorttitle = {Fertilization modes and the evolution of sperm characteristics in marine fishes},\n\turl = {https://onlinelibrary.wiley.com/doi/10.1002/ece3.9562},\n\tdoi = {10.1002/ece3.9562},\n\tlanguage = {en},\n\tnumber = {12},\n\turldate = {2022-12-07},\n\tjournal = {Ecology and Evolution},\n\tauthor = {Ito, Takeshi and Morita, Masaya and Okuno, Seiya and Inaba, Kazuo and Shiba, Kogiku and Munehara, Hiroyuki and Koya, Yasunori and Homma, Mitsuo and Awata, Satoshi},\n\tmonth = dec,\n\tyear = {2022},\n}\n\n</pre>\n</div>\n\n\n\n</div>\n\n\n  <div class=\"bibbase_paper\" id=\"jokura-sato-shiba-inaba-twodistinctcompartmentsofactenophorecombplateprovidestructuralandfunctionalintegrityforthemotilityofgiantmulticilia-2022\">\n  <span class=\"bibbase_paper_titleauthoryear\">\n\n  \n  <span class=\"bibbase_paper_author\">\n  Jokura, K., Sato, Y., Shiba, K.,  &amp; <a class=\"bibbase author link\" href=\"https://www.shimoda.tsukuba.ac.jp/en/staff_inaba.html\">Inaba, K.</a>\n</span>\n\n  <span class=\"bibbase_paper_title\">\n  \n  \n  \n  <a href=\"https://linkinghub.elsevier.com/retrieve/pii/S0960982222016037\"\n     onclick=\"return trackOutboundLink('publications', 'click', 'jokura-sato-shiba-inaba-twodistinctcompartmentsofactenophorecombplateprovidestructuralandfunctionalintegrityforthemotilityofgiantmulticilia-2022', 'https://linkinghub.elsevier.com/retrieve/pii/S0960982222016037', event);\">\n    Two distinct compartments of a ctenophore comb plate provide structural and functional integrity for the motility of giant multicilia.\n  </a>\n  \n  \n  \n</span>\n\n  \n\n</span>\n\n  <i>Current Biology</i>,S0960982222016037. October 2022.\n  <span class=\"note\"></span>\n\n<span class=\"bibbase_note\"></span>\n\n<br class=\"bibbase_paper_content\"/>\n\n<span class=\"bibbase_paper_content dontprint\">\n\n  \n  <a href=\"https://linkinghub.elsevier.com/retrieve/pii/S0960982222016037\"\n     onclick=\"log_download('jokura-sato-shiba-inaba-twodistinctcompartmentsofactenophorecombplateprovidestructuralandfunctionalintegrityforthemotilityofgiantmulticilia-2022', 'https://linkinghub.elsevier.com/retrieve/pii/S0960982222016037', event.ctrlKey, event)\">\n    <img src=\"//bibbase.org/img/filetypes/link.svg\"\n\t     alt=\"Two distinct compartments of a ctenophore comb plate provide structural and functional integrity for the motility of giant multicilia [link]\"\n       title=\"Paper\"\n\t     class=\"bibbase_icon\"\n         ><span class=\"bibbase_icon_text\">Paper</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"http://doi.org/10.1016/j.cub.2022.09.061\"\n     onclick=\"log_download('jokura-sato-shiba-inaba-twodistinctcompartmentsofactenophorecombplateprovidestructuralandfunctionalintegrityforthemotilityofgiantmulticilia-2022', 'http://doi.org/10.1016/j.cub.2022.09.061', event.ctrlKey)\"\n     class=\"bibbase doi link\">\n    <span>doi</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"//bibbase.org/network/publication/jokura-sato-shiba-inaba-twodistinctcompartmentsofactenophorecombplateprovidestructuralandfunctionalintegrityforthemotilityofgiantmulticilia-2022\"\n     class=\"bibbase bibbase_page link\">link</a>\n  &nbsp;\n  \n\n  <a href=\"#\"\n     onclick=\"showBib(event); return false;\"\n     class=\"bibbase bibtex link\">bibtex\n    <i class=\"fa fa-caret-down\"></i></a>\n\n  \n\n  \n\n  <!-- <a class=\"bibbase read link hidden\" -->\n  <!--    href=\"javascript:toggleRead('jokura-sato-shiba-inaba-twodistinctcompartmentsofactenophorecombplateprovidestructuralandfunctionalintegrityforthemotilityofgiantmulticilia-2022')\" -->\n  <!--    title=\"Marking this paper as read adds it to your library on BibBase.\" -->\n  <!--    > -->\n  <!--   <i class=\"fa fa-check\"></i>&nbsp; -->\n  <!--   <span>mark as read</span> -->\n  <!-- </a> -->\n\n  &nbsp;\n  \n  \n\n</span>\n\n<div class=\"well well-small bibbase\" data-type=\"bibtex\"\n     style=\"display:none\">\n  <pre style=\"white-space: pre-wrap;\">@article{jokura_two_2022,\n\ttitle = {Two distinct compartments of a ctenophore comb plate provide structural and functional integrity for the motility of giant multicilia},\n\tissn = {09609822},\n\turl = {https://linkinghub.elsevier.com/retrieve/pii/S0960982222016037},\n\tdoi = {10.1016/j.cub.2022.09.061},\n\tlanguage = {en},\n\turldate = {2022-10-25},\n\tjournal = {Current Biology},\n\tauthor = {Jokura, Kei and Sato, Yu and Shiba, Kogiku and Inaba, Kazuo},\n\tmonth = oct,\n\tyear = {2022},\n\tpages = {S0960982222016037},\n}\n\n</pre>\n</div>\n\n\n\n</div>\n\n\n  <div class=\"bibbase_paper\" id=\"nakano-isowa-inaba-jambioanditscoastalorganismjointsurveysnetworkofmarinestationsexploresjapanesecoastalbiota-2022\">\n  <span class=\"bibbase_paper_titleauthoryear\">\n\n  \n  <span class=\"bibbase_paper_author\">\n  Nakano, H., Isowa, Y.,  &amp; <a class=\"bibbase author link\" href=\"https://www.shimoda.tsukuba.ac.jp/en/staff_inaba.html\">Inaba, K.</a>\n</span>\n\n  <span class=\"bibbase_paper_title\">\n  \n  \n  \n  <a href=\"https://bioone.org/journals/zoological-science/volume-39/issue-1/zs210069/JAMBIO-and-Its-Coastal-Organism-Joint-Surveys--Network-of/10.2108/zs210069.full\"\n     onclick=\"return trackOutboundLink('publications', 'click', 'nakano-isowa-inaba-jambioanditscoastalorganismjointsurveysnetworkofmarinestationsexploresjapanesecoastalbiota-2022', 'https://bioone.org/journals/zoological-science/volume-39/issue-1/zs210069/JAMBIO-and-Its-Coastal-Organism-Joint-Surveys--Network-of/10.2108/zs210069.full', event);\">\n    JAMBIO and its coastal organism joint surveys: Network of marine stations explores Japanese coastal biota.\n  </a>\n  \n  \n  \n</span>\n\n  \n\n</span>\n\n  <i>Zoological Science</i>, 39(1): 1–6. February 2022.\n  <span class=\"note\"></span>\n\n<span class=\"bibbase_note\"></span>\n\n<br class=\"bibbase_paper_content\"/>\n\n<span class=\"bibbase_paper_content dontprint\">\n\n  \n  <a href=\"https://bioone.org/journals/zoological-science/volume-39/issue-1/zs210069/JAMBIO-and-Its-Coastal-Organism-Joint-Surveys--Network-of/10.2108/zs210069.full\"\n     onclick=\"log_download('nakano-isowa-inaba-jambioanditscoastalorganismjointsurveysnetworkofmarinestationsexploresjapanesecoastalbiota-2022', 'https://bioone.org/journals/zoological-science/volume-39/issue-1/zs210069/JAMBIO-and-Its-Coastal-Organism-Joint-Surveys--Network-of/10.2108/zs210069.full', event.ctrlKey, event)\">\n    <img src=\"//bibbase.org/img/filetypes/link.svg\"\n\t     alt=\"JAMBIO and its coastal organism joint surveys: Network of marine stations explores Japanese coastal biota [link]\"\n       title=\"Paper\"\n\t     class=\"bibbase_icon\"\n         ><span class=\"bibbase_icon_text\">Paper</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"http://doi.org/10.2108/zs210069\"\n     onclick=\"log_download('nakano-isowa-inaba-jambioanditscoastalorganismjointsurveysnetworkofmarinestationsexploresjapanesecoastalbiota-2022', 'http://doi.org/10.2108/zs210069', event.ctrlKey)\"\n     class=\"bibbase doi link\">\n    <span>doi</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"//bibbase.org/network/publication/nakano-isowa-inaba-jambioanditscoastalorganismjointsurveysnetworkofmarinestationsexploresjapanesecoastalbiota-2022\"\n     class=\"bibbase bibbase_page link\">link</a>\n  &nbsp;\n  \n\n  <a href=\"#\"\n     onclick=\"showBib(event); return false;\"\n     class=\"bibbase bibtex link\">bibtex\n    <i class=\"fa fa-caret-down\"></i></a>\n\n  \n\n  \n  &nbsp;\n  <span class=\"bibbase_stats_paper\" style=\"color: #777;\">\n    <span>30 downloads</span>\n  </span>\n  \n\n  <!-- <a class=\"bibbase read link hidden\" -->\n  <!--    href=\"javascript:toggleRead('nakano-isowa-inaba-jambioanditscoastalorganismjointsurveysnetworkofmarinestationsexploresjapanesecoastalbiota-2022')\" -->\n  <!--    title=\"Marking this paper as read adds it to your library on BibBase.\" -->\n  <!--    > -->\n  <!--   <i class=\"fa fa-check\"></i>&nbsp; -->\n  <!--   <span>mark as read</span> -->\n  <!-- </a> -->\n\n  &nbsp;\n  \n  \n\n</span>\n\n<div class=\"well well-small bibbase\" data-type=\"bibtex\"\n     style=\"display:none\">\n  <pre style=\"white-space: pre-wrap;\">@article{nakano_jambio_2022,\n\ttitle = {{JAMBIO} and its coastal organism joint surveys: {Network} of marine stations explores {Japanese} coastal biota},\n\tvolume = {39},\n\tissn = {0289-0003},\n\tshorttitle = {{JAMBIO} and {Its} {Coastal} {Organism} {Joint} {Surveys}},\n\turl = {https://bioone.org/journals/zoological-science/volume-39/issue-1/zs210069/JAMBIO-and-Its-Coastal-Organism-Joint-Surveys--Network-of/10.2108/zs210069.full},\n\tdoi = {10.2108/zs210069},\n\tnumber = {1},\n\turldate = {2022-02-04},\n\tjournal = {Zoological Science},\n\tauthor = {Nakano, Hiroaki and Isowa, Yukinobu and Inaba, Kazuo},\n\tmonth = feb,\n\tyear = {2022},\n\tpages = {1--6},\n}\n\n</pre>\n</div>\n\n\n\n</div>\n\n\n  <div class=\"bibbase_paper\" id=\"shiba-inaba-therolesoftwocngchannelsintheregulationofascidianspermchemotaxis-2022\">\n  <span class=\"bibbase_paper_titleauthoryear\">\n\n  \n  <span class=\"bibbase_paper_author\">\n  Shiba, K.,  &amp; <a class=\"bibbase author link\" href=\"https://www.shimoda.tsukuba.ac.jp/en/staff_inaba.html\">Inaba, K.</a>\n</span>\n\n  <span class=\"bibbase_paper_title\">\n  \n  \n  \n  <a href=\"https://www.mdpi.com/1422-0067/23/3/1648\"\n     onclick=\"return trackOutboundLink('publications', 'click', 'shiba-inaba-therolesoftwocngchannelsintheregulationofascidianspermchemotaxis-2022', 'https://www.mdpi.com/1422-0067/23/3/1648', event);\">\n    The roles of two CNG channels in the regulation of ascidian sperm chemotaxis.\n  </a>\n  \n  \n  \n</span>\n\n  \n\n</span>\n\n  <i>International Journal of Molecular Sciences</i>, 23(3): 1648. January 2022.\n  <span class=\"note\"></span>\n\n<span class=\"bibbase_note\"></span>\n\n<br class=\"bibbase_paper_content\"/>\n\n<span class=\"bibbase_paper_content dontprint\">\n\n  \n  <a href=\"https://www.mdpi.com/1422-0067/23/3/1648\"\n     onclick=\"log_download('shiba-inaba-therolesoftwocngchannelsintheregulationofascidianspermchemotaxis-2022', 'https://www.mdpi.com/1422-0067/23/3/1648', event.ctrlKey, event)\">\n    <img src=\"//bibbase.org/img/filetypes/link.svg\"\n\t     alt=\"The roles of two CNG channels in the regulation of ascidian sperm chemotaxis [link]\"\n       title=\"Paper\"\n\t     class=\"bibbase_icon\"\n         ><span class=\"bibbase_icon_text\">Paper</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"http://doi.org/10.3390/ijms23031648\"\n     onclick=\"log_download('shiba-inaba-therolesoftwocngchannelsintheregulationofascidianspermchemotaxis-2022', 'http://doi.org/10.3390/ijms23031648', event.ctrlKey)\"\n     class=\"bibbase doi link\">\n    <span>doi</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"//bibbase.org/network/publication/shiba-inaba-therolesoftwocngchannelsintheregulationofascidianspermchemotaxis-2022\"\n     class=\"bibbase bibbase_page link\">link</a>\n  &nbsp;\n  \n\n  <a href=\"#\"\n     onclick=\"showBib(event); return false;\"\n     class=\"bibbase bibtex link\">bibtex\n    <i class=\"fa fa-caret-down\"></i></a>\n\n  \n  &nbsp;\n  <a class=\"bibbase_abstract_link bibbase link\"\n     href=\"#\"\n     onclick=\"showAbstract(event); return false;\">\n    abstract <i class=\"fa fa-caret-down\"></i></a>\n  \n\n  \n  &nbsp;\n  <span class=\"bibbase_stats_paper\" style=\"color: #777;\">\n    <span>18 downloads</span>\n  </span>\n  \n\n  <!-- <a class=\"bibbase read link hidden\" -->\n  <!--    href=\"javascript:toggleRead('shiba-inaba-therolesoftwocngchannelsintheregulationofascidianspermchemotaxis-2022')\" -->\n  <!--    title=\"Marking this paper as read adds it to your library on BibBase.\" -->\n  <!--    > -->\n  <!--   <i class=\"fa fa-check\"></i>&nbsp; -->\n  <!--   <span>mark as read</span> -->\n  <!-- </a> -->\n\n  &nbsp;\n  \n  \n\n</span>\n\n<div class=\"well well-small bibbase\" data-type=\"bibtex\"\n     style=\"display:none\">\n  <pre style=\"white-space: pre-wrap;\">@article{shiba_roles_2022,\n\ttitle = {The roles of two {CNG} channels in the regulation of ascidian sperm chemotaxis},\n\tvolume = {23},\n\tissn = {1422-0067},\n\turl = {https://www.mdpi.com/1422-0067/23/3/1648},\n\tdoi = {10.3390/ijms23031648},\n\tabstract = {Spermatozoa sense and respond to their environmental signals to ensure fertilization success. Reception and transduction of signals are reflected rapidly in sperm flagellar waveforms and swimming behavior. In the ascidian Ciona intestinalis (type A; also called C. robusta), an egg-derived sulfated steroid called SAAF (sperm activating and attracting factor), induces both sperm motility activation and chemotaxis. Two types of CNG (cyclic nucleotide-gated) channels, Ci-tetra KCNG (tetrameric, cyclic nucleotide-gated, K+-selective) and Ci-HCN (hyperpolarization-activated and cyclic nucleotide-gated), are highly expressed in Ciona testis from the comprehensive gene expression analysis. To elucidate the sperm signaling pathway to regulate flagellar motility, we focus on the role of CNG channels. In this study, the immunochemical analysis revealed that both CNG channels are expressed in Ciona sperm and localized to sperm flagella. Sperm motility analysis and Ca2+ imaging during chemotaxis showed that CNG channel inhibition affected the changes in flagellar waveforms and Ca2+ efflux needed for the chemotactic turn. These results suggest that CNG channels in Ciona sperm play a vital role in regulating sperm motility and intracellular Ca2+ regulation during chemotaxis.},\n\tlanguage = {en},\n\tnumber = {3},\n\turldate = {2022-02-07},\n\tjournal = {International Journal of Molecular Sciences},\n\tauthor = {Shiba, Kogiku and Inaba, Kazuo},\n\tmonth = jan,\n\tyear = {2022},\n\tpages = {1648},\n}\n\n</pre>\n</div>\n\n\n<div class=\"well well-small bibbase\" data-type=\"abstract\"\n     style=\"display:none\">\n  Spermatozoa sense and respond to their environmental signals to ensure fertilization success. Reception and transduction of signals are reflected rapidly in sperm flagellar waveforms and swimming behavior. In the ascidian Ciona intestinalis (type A; also called C. robusta), an egg-derived sulfated steroid called SAAF (sperm activating and attracting factor), induces both sperm motility activation and chemotaxis. Two types of CNG (cyclic nucleotide-gated) channels, Ci-tetra KCNG (tetrameric, cyclic nucleotide-gated, K+-selective) and Ci-HCN (hyperpolarization-activated and cyclic nucleotide-gated), are highly expressed in Ciona testis from the comprehensive gene expression analysis. To elucidate the sperm signaling pathway to regulate flagellar motility, we focus on the role of CNG channels. In this study, the immunochemical analysis revealed that both CNG channels are expressed in Ciona sperm and localized to sperm flagella. Sperm motility analysis and Ca2+ imaging during chemotaxis showed that CNG channel inhibition affected the changes in flagellar waveforms and Ca2+ efflux needed for the chemotactic turn. These results suggest that CNG channels in Ciona sperm play a vital role in regulating sperm motility and intracellular Ca2+ regulation during chemotaxis.\n</div>\n\n\n</div>\n\n\n  <div class=\"bibbase_paper\" id=\"shibata-morita-sato-shiba-kitanobo-yokoya-inaba-axonemalgrowthandalignmentduringparaspermatogenesisinthemarinegastropodistrombusluhuanusi-2022\">\n  <span class=\"bibbase_paper_titleauthoryear\">\n\n  \n  <span class=\"bibbase_paper_author\">\n  Shibata, D., Morita, M., Sato, Y., Shiba, K., Kitanobo, S., Yokoya, R.,  &amp; <a class=\"bibbase author link\" href=\"https://www.shimoda.tsukuba.ac.jp/en/staff_inaba.html\">Inaba, K.</a>\n</span>\n\n  <span class=\"bibbase_paper_title\">\n  \n  \n  \n  <a href=\"https://www.frontiersin.org/articles/10.3389/fcell.2022.905748/full\"\n     onclick=\"return trackOutboundLink('publications', 'click', 'shibata-morita-sato-shiba-kitanobo-yokoya-inaba-axonemalgrowthandalignmentduringparaspermatogenesisinthemarinegastropodistrombusluhuanusi-2022', 'https://www.frontiersin.org/articles/10.3389/fcell.2022.905748/full', event);\">\n    Axonemal growth and alignment during paraspermatogenesis in the marine gastropod <i>Strombus luhuanus</i>.\n  </a>\n  \n  \n  \n</span>\n\n  \n\n</span>\n\n  <i>Frontiers in Cell and Developmental Biology</i>, 10: 905748. June 2022.\n  <span class=\"note\"></span>\n\n<span class=\"bibbase_note\"></span>\n\n<br class=\"bibbase_paper_content\"/>\n\n<span class=\"bibbase_paper_content dontprint\">\n\n  \n  <a href=\"https://www.frontiersin.org/articles/10.3389/fcell.2022.905748/full\"\n     onclick=\"log_download('shibata-morita-sato-shiba-kitanobo-yokoya-inaba-axonemalgrowthandalignmentduringparaspermatogenesisinthemarinegastropodistrombusluhuanusi-2022', 'https://www.frontiersin.org/articles/10.3389/fcell.2022.905748/full', event.ctrlKey, event)\">\n    <img src=\"//bibbase.org/img/filetypes/link.svg\"\n\t     alt=\"Axonemal growth and alignment during paraspermatogenesis in the marine gastropod &lt;i&gt;Strombus luhuanus&lt;/i&gt; [link]\"\n       title=\"Paper\"\n\t     class=\"bibbase_icon\"\n         ><span class=\"bibbase_icon_text\">Paper</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"http://doi.org/10.3389/fcell.2022.905748\"\n     onclick=\"log_download('shibata-morita-sato-shiba-kitanobo-yokoya-inaba-axonemalgrowthandalignmentduringparaspermatogenesisinthemarinegastropodistrombusluhuanusi-2022', 'http://doi.org/10.3389/fcell.2022.905748', event.ctrlKey)\"\n     class=\"bibbase doi link\">\n    <span>doi</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"//bibbase.org/network/publication/shibata-morita-sato-shiba-kitanobo-yokoya-inaba-axonemalgrowthandalignmentduringparaspermatogenesisinthemarinegastropodistrombusluhuanusi-2022\"\n     class=\"bibbase bibbase_page link\">link</a>\n  &nbsp;\n  \n\n  <a href=\"#\"\n     onclick=\"showBib(event); return false;\"\n     class=\"bibbase bibtex link\">bibtex\n    <i class=\"fa fa-caret-down\"></i></a>\n\n  \n  &nbsp;\n  <a class=\"bibbase_abstract_link bibbase link\"\n     href=\"#\"\n     onclick=\"showAbstract(event); return false;\">\n    abstract <i class=\"fa fa-caret-down\"></i></a>\n  \n\n  \n  &nbsp;\n  <span class=\"bibbase_stats_paper\" style=\"color: #777;\">\n    <span>18 downloads</span>\n  </span>\n  \n\n  <!-- <a class=\"bibbase read link hidden\" -->\n  <!--    href=\"javascript:toggleRead('shibata-morita-sato-shiba-kitanobo-yokoya-inaba-axonemalgrowthandalignmentduringparaspermatogenesisinthemarinegastropodistrombusluhuanusi-2022')\" -->\n  <!--    title=\"Marking this paper as read adds it to your library on BibBase.\" -->\n  <!--    > -->\n  <!--   <i class=\"fa fa-check\"></i>&nbsp; -->\n  <!--   <span>mark as read</span> -->\n  <!-- </a> -->\n\n  &nbsp;\n  \n  \n\n</span>\n\n<div class=\"well well-small bibbase\" data-type=\"bibtex\"\n     style=\"display:none\">\n  <pre style=\"white-space: pre-wrap;\">@article{shibata_axonemal_2022,\n\ttitle = {Axonemal growth and alignment during paraspermatogenesis in the marine gastropod \\textit{{Strombus} luhuanus}},\n\tvolume = {10},\n\tissn = {2296-634X},\n\turl = {https://www.frontiersin.org/articles/10.3389/fcell.2022.905748/full},\n\tdoi = {10.3389/fcell.2022.905748},\n\tabstract = {Parasperm are non-fertilizing sperm that are produced simultaneously with fertile eusperm. They occur in several animal species and show considerable morphological diversity. We investigated the dynamics of axonemes during paraspermatogenesis in the marine snail\n              S. luhuanus\n              . Mature parasperm were characterized by two lateral undulating membranes for motility and many globular vesicles. Axonemes were first observed as brush-like structures that extruded from the anterior region. Multiple axonemes longer than the brush then started to extend inside the cytoplasm towards the posterior region. The mass of the axonemes separated into two lateral rows and formed an undulating membrane that drives bidirectional swimming in the mature parasperm. The central pair of axonemes was aligned in the undulating membrane, resulting in cooperative bend propagation. During paraspermatogenesis, centrioles were largely diminished and localized to the anterior region. CEP290, a major component of the transition zone, showed a broad distribution in the anterior area. Axonemes in the posterior region showed a 9 + 0 structure with both outer and inner arm dyneins. These observations provide a structural basis for understanding the physiological functions of parasperm in marine reproductive strategies.},\n\turldate = {2022-07-12},\n\tjournal = {Frontiers in Cell and Developmental Biology},\n\tauthor = {Shibata, Daisuke and Morita, Masaya and Sato, Yu and Shiba, Kogiku and Kitanobo, Seiya and Yokoya, Ryo and Inaba, Kazuo},\n\tmonth = jun,\n\tyear = {2022},\n\tpages = {905748},\n}\n\n</pre>\n</div>\n\n\n<div class=\"well well-small bibbase\" data-type=\"abstract\"\n     style=\"display:none\">\n  Parasperm are non-fertilizing sperm that are produced simultaneously with fertile eusperm. They occur in several animal species and show considerable morphological diversity. We investigated the dynamics of axonemes during paraspermatogenesis in the marine snail S. luhuanus . Mature parasperm were characterized by two lateral undulating membranes for motility and many globular vesicles. Axonemes were first observed as brush-like structures that extruded from the anterior region. Multiple axonemes longer than the brush then started to extend inside the cytoplasm towards the posterior region. The mass of the axonemes separated into two lateral rows and formed an undulating membrane that drives bidirectional swimming in the mature parasperm. The central pair of axonemes was aligned in the undulating membrane, resulting in cooperative bend propagation. During paraspermatogenesis, centrioles were largely diminished and localized to the anterior region. CEP290, a major component of the transition zone, showed a broad distribution in the anterior area. Axonemes in the posterior region showed a 9 + 0 structure with both outer and inner arm dyneins. These observations provide a structural basis for understanding the physiological functions of parasperm in marine reproductive strategies.\n</div>\n\n\n</div>\n\n\n  <div class=\"bibbase_paper\" id=\"sugiura-shiba-inaba-matsumoto-morphologicaldifferencesintardigradespermatozoainducevariationingametemotility-2022\">\n  <span class=\"bibbase_paper_titleauthoryear\">\n\n  \n  <span class=\"bibbase_paper_author\">\n  Sugiura, K., Shiba, K., <a class=\"bibbase author link\" href=\"https://www.shimoda.tsukuba.ac.jp/en/staff_inaba.html\">Inaba, K.</a>,  &amp; Matsumoto, M.\n</span>\n\n  <span class=\"bibbase_paper_title\">\n  \n  \n  \n  <a href=\"https:///bmczool.biomedcentral.com/articles/10.1186/s40850-022-00109-w\"\n     onclick=\"return trackOutboundLink('publications', 'click', 'sugiura-shiba-inaba-matsumoto-morphologicaldifferencesintardigradespermatozoainducevariationingametemotility-2022', 'https:///bmczool.biomedcentral.com/articles/10.1186/s40850-022-00109-w', event);\">\n    Morphological differences in tardigrade spermatozoa induce variation in gamete motility.\n  </a>\n  \n  \n  \n</span>\n\n  \n\n</span>\n\n  <i>BMC Zoology</i>, 7(1): 8. December 2022.\n  <span class=\"note\"></span>\n\n<span class=\"bibbase_note\"></span>\n\n<br class=\"bibbase_paper_content\"/>\n\n<span class=\"bibbase_paper_content dontprint\">\n\n  \n  <a href=\"https:///bmczool.biomedcentral.com/articles/10.1186/s40850-022-00109-w\"\n     onclick=\"log_download('sugiura-shiba-inaba-matsumoto-morphologicaldifferencesintardigradespermatozoainducevariationingametemotility-2022', 'https:///bmczool.biomedcentral.com/articles/10.1186/s40850-022-00109-w', event.ctrlKey, event)\">\n    <img src=\"//bibbase.org/img/filetypes/link.svg\"\n\t     alt=\"Morphological differences in tardigrade spermatozoa induce variation in gamete motility [link]\"\n       title=\"Paper\"\n\t     class=\"bibbase_icon\"\n         ><span class=\"bibbase_icon_text\">Paper</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"http://doi.org/10.1186/s40850-022-00109-w\"\n     onclick=\"log_download('sugiura-shiba-inaba-matsumoto-morphologicaldifferencesintardigradespermatozoainducevariationingametemotility-2022', 'http://doi.org/10.1186/s40850-022-00109-w', event.ctrlKey)\"\n     class=\"bibbase doi link\">\n    <span>doi</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"//bibbase.org/network/publication/sugiura-shiba-inaba-matsumoto-morphologicaldifferencesintardigradespermatozoainducevariationingametemotility-2022\"\n     class=\"bibbase bibbase_page link\">link</a>\n  &nbsp;\n  \n\n  <a href=\"#\"\n     onclick=\"showBib(event); return false;\"\n     class=\"bibbase bibtex link\">bibtex\n    <i class=\"fa fa-caret-down\"></i></a>\n\n  \n  &nbsp;\n  <a class=\"bibbase_abstract_link bibbase link\"\n     href=\"#\"\n     onclick=\"showAbstract(event); return false;\">\n    abstract <i class=\"fa fa-caret-down\"></i></a>\n  \n\n  \n  &nbsp;\n  <span class=\"bibbase_stats_paper\" style=\"color: #777;\">\n    <span>18 downloads</span>\n  </span>\n  \n\n  <!-- <a class=\"bibbase read link hidden\" -->\n  <!--    href=\"javascript:toggleRead('sugiura-shiba-inaba-matsumoto-morphologicaldifferencesintardigradespermatozoainducevariationingametemotility-2022')\" -->\n  <!--    title=\"Marking this paper as read adds it to your library on BibBase.\" -->\n  <!--    > -->\n  <!--   <i class=\"fa fa-check\"></i>&nbsp; -->\n  <!--   <span>mark as read</span> -->\n  <!-- </a> -->\n\n  &nbsp;\n  \n  \n\n</span>\n\n<div class=\"well well-small bibbase\" data-type=\"bibtex\"\n     style=\"display:none\">\n  <pre style=\"white-space: pre-wrap;\">@article{sugiura_morphological_2022,\n\ttitle = {Morphological differences in tardigrade spermatozoa induce variation in gamete motility},\n\tvolume = {7},\n\tissn = {2056-3132},\n\turl = {https:///bmczool.biomedcentral.com/articles/10.1186/s40850-022-00109-w},\n\tdoi = {10.1186/s40850-022-00109-w},\n\tabstract = {Abstract\n            \n              Background\n              \n                Fertilization is an event at the beginning of ontogeny. Successful fertilization depends on strategies for uniting female and male gametes that developed throughout evolutionary history. In some species of tardigrades, investigations of reproduction have revealed that released spermatozoa swim in the water to reach a female, after which the gametes are stored in her body. The morphology of the spermatozoa includes a coiled nucleus and a species-specific-length acrosome. Although the mating behaviour and morphology of tardigrades have been reported, the motility of male gametes remains unknown. Here, using a high-speed camera, we recorded the spermatozoon motilities of two tardigrades,\n                Paramacrobiotus\n                sp. and\n                Macrobiotus shonaicus,\n                which have longer and shorter spermatozoa, respectively.\n              \n            \n            \n              Results\n              \n                The movement of spermatozoa was faster in\n                Paramacrobiotus\n                sp. than in\n                M. shonaicus\n                , but the beat frequencies of the tails were equal, suggesting that the long tail improved acceleration. In both species, the head part consisting of a coiled nucleus and an acrosome did not swing, in contrast to the tail. The head part of\n                Paramacrobiotus\n                sp. spermatozoa swung harder during turning; in contrast, the tail of\n                M. shonaicus\n                moved more widely than the head. Finally, after mating, the spermatozoa that reached the female aggregated around the cloaca while waiting to enter her body in both tested species.\n              \n            \n            \n              Conclusions\n              \n                This study provides results for the first observations and analyses of individual spermatozoon motility in tardigrades. A comparison of the spermatozoon movements of the two tardigrades suggested that the motilities of the male gametes were affected by morphological differences, where the longer spermatozoa swam faster and the shorter ones showed more stable swimming. Swimming was mainly induced by tail movement, but the long head of\n                Paramacrobiotus\n                sp. spermatozoa might be especially important for turning. In addition, observations of mated female cloacae suggested that the head parts of the spermatozoa were required for aggregation around the cloaca of a mated female.},\n\tlanguage = {en},\n\tnumber = {1},\n\turldate = {2022-02-07},\n\tjournal = {BMC Zoology},\n\tauthor = {Sugiura, Kenta and Shiba, Kogiku and Inaba, Kazuo and Matsumoto, Midori},\n\tmonth = dec,\n\tyear = {2022},\n\tpages = {8},\n}\n\n</pre>\n</div>\n\n\n<div class=\"well well-small bibbase\" data-type=\"abstract\"\n     style=\"display:none\">\n  Abstract Background Fertilization is an event at the beginning of ontogeny. Successful fertilization depends on strategies for uniting female and male gametes that developed throughout evolutionary history. In some species of tardigrades, investigations of reproduction have revealed that released spermatozoa swim in the water to reach a female, after which the gametes are stored in her body. The morphology of the spermatozoa includes a coiled nucleus and a species-specific-length acrosome. Although the mating behaviour and morphology of tardigrades have been reported, the motility of male gametes remains unknown. Here, using a high-speed camera, we recorded the spermatozoon motilities of two tardigrades, Paramacrobiotus sp. and Macrobiotus shonaicus, which have longer and shorter spermatozoa, respectively. Results The movement of spermatozoa was faster in Paramacrobiotus sp. than in M. shonaicus , but the beat frequencies of the tails were equal, suggesting that the long tail improved acceleration. In both species, the head part consisting of a coiled nucleus and an acrosome did not swing, in contrast to the tail. The head part of Paramacrobiotus sp. spermatozoa swung harder during turning; in contrast, the tail of M. shonaicus moved more widely than the head. Finally, after mating, the spermatozoa that reached the female aggregated around the cloaca while waiting to enter her body in both tested species. Conclusions This study provides results for the first observations and analyses of individual spermatozoon motility in tardigrades. A comparison of the spermatozoon movements of the two tardigrades suggested that the motilities of the male gametes were affected by morphological differences, where the longer spermatozoa swam faster and the shorter ones showed more stable swimming. Swimming was mainly induced by tail movement, but the long head of Paramacrobiotus sp. spermatozoa might be especially important for turning. In addition, observations of mated female cloacae suggested that the head parts of the spermatozoa were required for aggregation around the cloaca of a mated female.\n</div>\n\n\n</div>\n\n\n\n\n\n  </div>\n\n\n  <!-- Modal -->\n\n  <!-- <iframe id=\"bibbase_network_frame\" -->\n  <!--         src=\"//bibbase.org/network/control\" -->\n  <!--         style=\"display: none;\"> -->\n  <!-- </iframe> -->\n\n</div>\n"}; document.write(bibbase_data.data);